On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments.

2.50
Hdl Handle:
http://hdl.handle.net/10033/240838
Title:
On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments.
Authors:
Wargenau, Andreas; Fleissner, André; Bolten, Christoph Josef; Rohde, Manfred; Kampen, Ingo; Kwade, Arno
Abstract:
The electrostatic surface potential of fungal spores is generally regarded as potentially influencing spore aggregation and pellet formation in submerged cultures of filamentous fungi. Spores of Aspergillus niger are typically characterized by negative zeta potentials over a wide range of pH values. In this study, this particular behavior is ascribed to the presence of an extensive melanin coating. It is proposed on the basis of zeta potential and pigment extraction experiments that this outermost layer affects the pH-dependent surface potential in two manners: (i) by the addition of negative charges to the spore surface and (ii) by the pH-dependent release of melanin pigment. Chemical analyses revealed that deprotonation of melanin-bound carboxyl groups is most probably responsible for pigment release under acidic conditions. These findings were incorporated into a simple model which has the ability to qualitatively explain the results of zeta potential experiments and, moreover, to provide the basis for quantitative investigations on the role of electrostatics in spore aggregation.
Affiliation:
Institut für Partikeltechnik, Technische Universität Braunschweig, Volkmaroder Straße 5, D-38104 Braunschweig, Germany. wargenau@a-wargenau.de
Citation:
On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments. 2011, 162 (10):1011-7 Res. Microbiol.
Journal:
Research in microbiology
Issue Date:
Dec-2011
URI:
http://hdl.handle.net/10033/240838
DOI:
10.1016/j.resmic.2011.07.006
PubMed ID:
21835241
Type:
Article
Language:
en
ISSN:
1769-7123
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorWargenau, Andreasen_GB
dc.contributor.authorFleissner, Andréen_GB
dc.contributor.authorBolten, Christoph Josefen_GB
dc.contributor.authorRohde, Manfreden_GB
dc.contributor.authorKampen, Ingoen_GB
dc.contributor.authorKwade, Arnoen_GB
dc.date.accessioned2012-08-31T14:35:10Z-
dc.date.available2012-08-31T14:35:10Z-
dc.date.issued2011-12-
dc.identifier.citationOn the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments. 2011, 162 (10):1011-7 Res. Microbiol.en_GB
dc.identifier.issn1769-7123-
dc.identifier.pmid21835241-
dc.identifier.doi10.1016/j.resmic.2011.07.006-
dc.identifier.urihttp://hdl.handle.net/10033/240838-
dc.description.abstractThe electrostatic surface potential of fungal spores is generally regarded as potentially influencing spore aggregation and pellet formation in submerged cultures of filamentous fungi. Spores of Aspergillus niger are typically characterized by negative zeta potentials over a wide range of pH values. In this study, this particular behavior is ascribed to the presence of an extensive melanin coating. It is proposed on the basis of zeta potential and pigment extraction experiments that this outermost layer affects the pH-dependent surface potential in two manners: (i) by the addition of negative charges to the spore surface and (ii) by the pH-dependent release of melanin pigment. Chemical analyses revealed that deprotonation of melanin-bound carboxyl groups is most probably responsible for pigment release under acidic conditions. These findings were incorporated into a simple model which has the ability to qualitatively explain the results of zeta potential experiments and, moreover, to provide the basis for quantitative investigations on the role of electrostatics in spore aggregation.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Research in microbiologyen_GB
dc.subject.meshAspergillus nigeren_GB
dc.subject.meshEnvironmenten_GB
dc.subject.meshHydrogen-Ion Concentrationen_GB
dc.subject.meshMelaninsen_GB
dc.subject.meshMicroscopy, Electron, Transmissionen_GB
dc.subject.meshSpores, Fungalen_GB
dc.subject.meshStatic Electricityen_GB
dc.subject.meshSurface Propertiesen_GB
dc.titleOn the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments.en
dc.typeArticleen
dc.contributor.departmentInstitut für Partikeltechnik, Technische Universität Braunschweig, Volkmaroder Straße 5, D-38104 Braunschweig, Germany. wargenau@a-wargenau.deen_GB
dc.identifier.journalResearch in microbiologyen_GB

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