2.50
HDL Handle:
http://hdl.handle.net/10033/241697
Title:
LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes.
Authors:
Meier, Karin; Mathieu, Eve-Lyne; Finkernagel, Florian; Reuter, L Maximilian; Scharfe, Maren; Doehlemann, Gunther; Jarek, Michael; Brehm, Alexander
Abstract:
Mutations in the l(3)mbt tumour suppressor result in overproliferation of Drosophila larval brains. Recently, the derepression of different gene classes in l(3)mbt mutants was shown to be causal for transformation. However, the molecular mechanisms of dL(3)mbt-mediated gene repression are not understood. Here, we identify LINT, the major dL(3)mbt complex of Drosophila. LINT has three core subunits-dL(3)mbt, dCoREST, and dLint-1-and is expressed in cell lines, embryos, and larval brain. Using genome-wide ChIP-Seq analysis, we show that dLint-1 binds close to the TSS of tumour-relevant target genes. Depletion of the LINT core subunits results in derepression of these genes. By contrast, histone deacetylase, histone methylase, and histone demethylase activities are not required to maintain repression. Our results support a direct role of LINT in the repression of brain tumour-relevant target genes by restricting promoter access.
Affiliation:
Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.
Citation:
LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. 2012, 8 (5):e1002676 PLoS Genet.
Journal:
PLoS genetics
Issue Date:
May-2012
URI:
http://hdl.handle.net/10033/241697
DOI:
10.1371/journal.pgen.1002676
PubMed ID:
22570633
Type:
Article
Language:
en
ISSN:
1553-7404
Appears in Collections:
Publikations of the AG Genomanalytik(GMAK)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMeier, Karinen_GB
dc.contributor.authorMathieu, Eve-Lyneen_GB
dc.contributor.authorFinkernagel, Florianen_GB
dc.contributor.authorReuter, L Maximilianen_GB
dc.contributor.authorScharfe, Marenen_GB
dc.contributor.authorDoehlemann, Guntheren_GB
dc.contributor.authorJarek, Michaelen_GB
dc.contributor.authorBrehm, Alexanderen_GB
dc.date.accessioned2012-09-06T11:17:24Z-
dc.date.available2012-09-06T11:17:24Z-
dc.date.issued2012-05-
dc.identifier.citationLINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. 2012, 8 (5):e1002676 PLoS Genet.en_GB
dc.identifier.issn1553-7404-
dc.identifier.pmid22570633-
dc.identifier.doi10.1371/journal.pgen.1002676-
dc.identifier.urihttp://hdl.handle.net/10033/241697-
dc.description.abstractMutations in the l(3)mbt tumour suppressor result in overproliferation of Drosophila larval brains. Recently, the derepression of different gene classes in l(3)mbt mutants was shown to be causal for transformation. However, the molecular mechanisms of dL(3)mbt-mediated gene repression are not understood. Here, we identify LINT, the major dL(3)mbt complex of Drosophila. LINT has three core subunits-dL(3)mbt, dCoREST, and dLint-1-and is expressed in cell lines, embryos, and larval brain. Using genome-wide ChIP-Seq analysis, we show that dLint-1 binds close to the TSS of tumour-relevant target genes. Depletion of the LINT core subunits results in derepression of these genes. By contrast, histone deacetylase, histone methylase, and histone demethylase activities are not required to maintain repression. Our results support a direct role of LINT in the repression of brain tumour-relevant target genes by restricting promoter access.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PLoS geneticsen_GB
dc.titleLINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes.en
dc.typeArticleen
dc.contributor.departmentInstitut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.en_GB
dc.identifier.journalPLoS geneticsen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.