Analysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships.

2.50
Hdl Handle:
http://hdl.handle.net/10033/244592
Title:
Analysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships.
Authors:
Brettar, Ingrid; Christen, Richard; Höfle, Manfred G
Abstract:
Understanding structure-function links of microbial communities is a central theme of microbial ecology since its beginning. To this end, we studied the spatial variability of the bacterioplankton community structure and composition across the central Baltic Sea at four stations, which were up to 450 km apart and at a depth profile representative for the central part (Gotland Deep, 235 m). Bacterial community structure was followed by 16S ribosomal RNA (rRNA)- and 16S rRNA gene-based fingerprints using single-strand conformation polymorphism (SSCP) electrophoresis. Species composition was determined by sequence analysis of SSCP bands. High similarities of the bacterioplankton communities across several hundred kilometers were observed in the surface water using RNA- and DNA-based fingerprints. In these surface communities, the RNA- and DNA-based fingerprints resulted in very different pattern, presumably indicating large difference between the active members of the community as represented by RNA-based fingerprints and the present members represented by the DNA-based fingerprints. This large discrepancy changed gradually over depth, resulting in highly similar RNA- and DNA-based fingerprints in the anoxic part of the water column below 130 m depth. A conceivable mechanism explaining this high similarity could be the reduced oxidative stress in the anoxic zone. The stable communities on the surface and in the anoxic zone indicate the strong influence of the hydrography on the bacterioplankton community structure. Comparative analysis of RNA- and DNA-based community structure provided criteria for the identification of the core community, its key members and their links to biogeochemical functions.
Affiliation:
Department of Vaccinology and Applied Microbiology, Helmholtz Centre of Infection Research (HZI), Braunschweig, Germany.
Citation:
Analysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships. 2012, 6 (1):195-212 ISME J
Journal:
The ISME journal
Issue Date:
Jan-2012
URI:
http://hdl.handle.net/10033/244592
DOI:
10.1038/ismej.2011.80
PubMed ID:
21697960
Type:
Article
Language:
en
ISSN:
1751-7370
Appears in Collections:
Publications of Dept. Vaccinology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorBrettar, Ingriden_GB
dc.contributor.authorChristen, Richarden_GB
dc.contributor.authorHöfle, Manfred Gen_GB
dc.date.accessioned2012-09-18T08:34:00Z-
dc.date.available2012-09-18T08:34:00Z-
dc.date.issued2012-01-
dc.identifier.citationAnalysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships. 2012, 6 (1):195-212 ISME Jen_GB
dc.identifier.issn1751-7370-
dc.identifier.pmid21697960-
dc.identifier.doi10.1038/ismej.2011.80-
dc.identifier.urihttp://hdl.handle.net/10033/244592-
dc.description.abstractUnderstanding structure-function links of microbial communities is a central theme of microbial ecology since its beginning. To this end, we studied the spatial variability of the bacterioplankton community structure and composition across the central Baltic Sea at four stations, which were up to 450 km apart and at a depth profile representative for the central part (Gotland Deep, 235 m). Bacterial community structure was followed by 16S ribosomal RNA (rRNA)- and 16S rRNA gene-based fingerprints using single-strand conformation polymorphism (SSCP) electrophoresis. Species composition was determined by sequence analysis of SSCP bands. High similarities of the bacterioplankton communities across several hundred kilometers were observed in the surface water using RNA- and DNA-based fingerprints. In these surface communities, the RNA- and DNA-based fingerprints resulted in very different pattern, presumably indicating large difference between the active members of the community as represented by RNA-based fingerprints and the present members represented by the DNA-based fingerprints. This large discrepancy changed gradually over depth, resulting in highly similar RNA- and DNA-based fingerprints in the anoxic part of the water column below 130 m depth. A conceivable mechanism explaining this high similarity could be the reduced oxidative stress in the anoxic zone. The stable communities on the surface and in the anoxic zone indicate the strong influence of the hydrography on the bacterioplankton community structure. Comparative analysis of RNA- and DNA-based community structure provided criteria for the identification of the core community, its key members and their links to biogeochemical functions.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The ISME journalen_GB
dc.subject.meshBacteriaen_GB
dc.subject.meshBaltic Statesen_GB
dc.subject.meshDNA Fingerprintingen_GB
dc.subject.meshDNA, Bacterialen_GB
dc.subject.meshGenes, rRNAen_GB
dc.subject.meshOceans and Seasen_GB
dc.subject.meshPhylogenyen_GB
dc.subject.meshPlanktonen_GB
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_GB
dc.subject.meshRNA, Ribosomal, 16Sen_GB
dc.subject.meshSeawateren_GB
dc.titleAnalysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships.en
dc.typeArticleen
dc.contributor.departmentDepartment of Vaccinology and Applied Microbiology, Helmholtz Centre of Infection Research (HZI), Braunschweig, Germany.en_GB
dc.identifier.journalThe ISME journalen_GB

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