Microevolution of pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions.

2.50
Hdl Handle:
http://hdl.handle.net/10033/245231
Title:
Microevolution of pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions.
Authors:
García, Katherine; Gavilán, Ronnie G; Höfle, Manfred G; Martínez-Urtaza, Jaime; Espejo, Romilio T
Abstract:
The emergence of the pandemic strain Vibrio parahaemolyticus O3:K6 in 1996 caused a large increase of diarrhea outbreaks related to seafood consumption in Southeast Asia, and later worldwide. Isolates of this strain constitutes a clonal complex, and their effectual differentiation is possible by comparison of their variable number tandem repeats (VNTRs). The differentiation of the isolates by the differences in VNTRs will allow inferring the population dynamics and microevolution of this strain but this requires knowing the rate and mechanism of VNTRs' variation. Our study of mutants obtained after serial cultivation of clones showed that mutation rates of the six VNTRs examined are on the order of 10(-4) mutant per generation and that difference increases by stepwise addition of single mutations. The single stepwise mutation (SSM) was deduced because mutants with 1, 2, 3, or more repeat unit deletions or insertions follow a geometric distribution. Plausible phylogenetic trees are obtained when, according to SSM, the genetic distance between clusters with different number of repeats is assessed by the absolute differences in repeats. Using this approach, mutants originated from different isolates of pandemic V. parahaemolyticus after serial cultivation are clustered with their parental isolates. Additionally, isolates of pandemic V. parahaemolyticus from Southeast Asia, Tokyo, and northern and southern Chile are clustered according their geographical origin. The deepest split in these four populations is observed between the Tokyo and southern Chile populations. We conclude that proper phylogenetic relations and successful tracing of pandemic V. parahaemolyticus requires measuring the differences between isolates by the absolute number of repeats in the VNTRs considered.
Affiliation:
Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.
Citation:
Microevolution of pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions. 2012, 7 (1):e30823 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/245231
DOI:
10.1371/journal.pone.0030823
PubMed ID:
22292049
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Vaccinology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGarcía, Katherineen_GB
dc.contributor.authorGavilán, Ronnie Gen_GB
dc.contributor.authorHöfle, Manfred Gen_GB
dc.contributor.authorMartínez-Urtaza, Jaimeen_GB
dc.contributor.authorEspejo, Romilio Ten_GB
dc.date.accessioned2012-09-20T11:17:16Z-
dc.date.available2012-09-20T11:17:16Z-
dc.date.issued2012-
dc.identifier.citationMicroevolution of pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions. 2012, 7 (1):e30823 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22292049-
dc.identifier.doi10.1371/journal.pone.0030823-
dc.identifier.urihttp://hdl.handle.net/10033/245231-
dc.description.abstractThe emergence of the pandemic strain Vibrio parahaemolyticus O3:K6 in 1996 caused a large increase of diarrhea outbreaks related to seafood consumption in Southeast Asia, and later worldwide. Isolates of this strain constitutes a clonal complex, and their effectual differentiation is possible by comparison of their variable number tandem repeats (VNTRs). The differentiation of the isolates by the differences in VNTRs will allow inferring the population dynamics and microevolution of this strain but this requires knowing the rate and mechanism of VNTRs' variation. Our study of mutants obtained after serial cultivation of clones showed that mutation rates of the six VNTRs examined are on the order of 10(-4) mutant per generation and that difference increases by stepwise addition of single mutations. The single stepwise mutation (SSM) was deduced because mutants with 1, 2, 3, or more repeat unit deletions or insertions follow a geometric distribution. Plausible phylogenetic trees are obtained when, according to SSM, the genetic distance between clusters with different number of repeats is assessed by the absolute differences in repeats. Using this approach, mutants originated from different isolates of pandemic V. parahaemolyticus after serial cultivation are clustered with their parental isolates. Additionally, isolates of pandemic V. parahaemolyticus from Southeast Asia, Tokyo, and northern and southern Chile are clustered according their geographical origin. The deepest split in these four populations is observed between the Tokyo and southern Chile populations. We conclude that proper phylogenetic relations and successful tracing of pandemic V. parahaemolyticus requires measuring the differences between isolates by the absolute number of repeats in the VNTRs considered.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAntigenic Variationen_GB
dc.subject.meshBacterial Typing Techniquesen_GB
dc.subject.meshDNA, Bacterialen_GB
dc.subject.meshEvolution, Molecularen_GB
dc.subject.meshGenetic Variationen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMicrosatellite Repeatsen_GB
dc.subject.meshMinisatellite Repeatsen_GB
dc.subject.meshMutation Rateen_GB
dc.subject.meshPandemicsen_GB
dc.subject.meshPhylogenyen_GB
dc.subject.meshVibrio Infectionsen_GB
dc.subject.meshVibrio parahaemolyticusen_GB
dc.titleMicroevolution of pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions.en
dc.typeArticleen
dc.contributor.departmentInstituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.en_GB
dc.identifier.journalPloS oneen_GB

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