Transcriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1.

2.50
Hdl Handle:
http://hdl.handle.net/10033/246971
Title:
Transcriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1.
Authors:
Gödeke, Julia; Binnenkade, Lucas; Thormann, Kai M
Abstract:
Bacterial biofilm formation starts with single cells attaching to a surface, however, little is known about the initial attachment steps and the adaptation to the surface-associated life style. Here, we describe a hydrodynamic system that allows easy harvest of cells at very early biofilm stages. Using the metal ion-reducing gammaproteobacterium Shewanella oneidensis MR-1 as a model organism, we analyzed the transcriptional changes occurring during surface-associated growth between 15 and 60 minutes after attachment. 230 genes were significantly upregulated and 333 were downregulated by a factor of ≥ 2. Main functional categories of the corresponding gene products comprise metabolism, uptake and transport, regulation, and hypothetical proteins. Among the genes highly upregulated those implicated in iron uptake are highly overrepresented, strongly indicating that S. oneidensis MR-1 has a high demand for iron during surface attachment and initial biofilm stages. Subsequent microscopic analysis of biofilm formation under hydrodynamic conditions revealed that addition of Fe(II) significantly stimulated biofilm formation of S. oneidensis MR-1 while planktonic growth was not affected. Our approach to harvest cells for transcriptional analysis of early biofilm stages is expected to be easily adapted to other bacterial species.
Affiliation:
Department of Ecophysiology, Max-Planck-Institut für terrestrische Mikrobiologie, Marburg, Germany.
Citation:
Transcriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1. 2012, 7 (7):e42160 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/246971
DOI:
10.1371/journal.pone.0042160
PubMed ID:
22860070
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of the TwinCore unit experimental Infectionresearch(EXPI)

Full metadata record

DC FieldValue Language
dc.contributor.authorGödeke, Juliaen_GB
dc.contributor.authorBinnenkade, Lucasen_GB
dc.contributor.authorThormann, Kai Men_GB
dc.date.accessioned2012-10-04T09:35:18Z-
dc.date.available2012-10-04T09:35:18Z-
dc.date.issued2012-
dc.identifier.citationTranscriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1. 2012, 7 (7):e42160 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22860070-
dc.identifier.doi10.1371/journal.pone.0042160-
dc.identifier.urihttp://hdl.handle.net/10033/246971-
dc.description.abstractBacterial biofilm formation starts with single cells attaching to a surface, however, little is known about the initial attachment steps and the adaptation to the surface-associated life style. Here, we describe a hydrodynamic system that allows easy harvest of cells at very early biofilm stages. Using the metal ion-reducing gammaproteobacterium Shewanella oneidensis MR-1 as a model organism, we analyzed the transcriptional changes occurring during surface-associated growth between 15 and 60 minutes after attachment. 230 genes were significantly upregulated and 333 were downregulated by a factor of ≥ 2. Main functional categories of the corresponding gene products comprise metabolism, uptake and transport, regulation, and hypothetical proteins. Among the genes highly upregulated those implicated in iron uptake are highly overrepresented, strongly indicating that S. oneidensis MR-1 has a high demand for iron during surface attachment and initial biofilm stages. Subsequent microscopic analysis of biofilm formation under hydrodynamic conditions revealed that addition of Fe(II) significantly stimulated biofilm formation of S. oneidensis MR-1 while planktonic growth was not affected. Our approach to harvest cells for transcriptional analysis of early biofilm stages is expected to be easily adapted to other bacterial species.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleTranscriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1.en
dc.typeArticleen
dc.contributor.departmentDepartment of Ecophysiology, Max-Planck-Institut für terrestrische Mikrobiologie, Marburg, Germany.en_GB
dc.identifier.journalPloS oneen_GB

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