2.50
HDL Handle:
http://hdl.handle.net/10033/246993
Title:
Characterisation of bovine leukocyte Ig-like receptors.
Authors:
Hogan, Louise; Bhuju, Sabin; Jones, Des C; Laing, Ken; Trowsdale, John; Butcher, Philip; Singh, Mahavir; Vordermeier, Martin; Allen, Rachel L
Abstract:
Leukocyte Immunoglobulin-like receptors (LILR) are innate immune receptors involved in regulating both innate and adaptive immune functions. LILR show more interspecies conservation than the closely related Killer Ig-like receptors, and homologues have been identified in rodents, primates, seals and chickens. The murine equivalents, paired Ig-like receptors (PIR), contain two additional immunoglobulin domains, but show strong sequence and functional similarities to human LILR. The bovine genome was recently sequenced, with preliminary annotations indicating that LILR were present in this species. We therefore sought to identify and characterize novel LILR within the Bos taurus genome, compare these phylogenetically with LILR from other species and determine whether they were expressed in vivo. Twenty six potential bovine LILR were initially identified using BLAST and BLAT software. Phylogenetic analysis constructed using the neighbour-joining method, incorporating pairwise deletion and confidence limits estimated from 1000 replicates using bootstrapping, indicated that 16 of these represent novel bovine LILR. Protein structures defined using protein BLAST predict that the bovine LILR family comprises seven putative inhibitory, four activating and five soluble receptors. Preliminary expression analysis was performed by mapping the predicted sequences with raw data from total transcript sequence generated using Genome Analyzer IIx (Illumina) to provide evidence that all 16 of these receptors are expressed in vivo. The bovine receptor family appears to contain receptors which resemble the six domain rodent PIR as well as the four domain LILR found in other species.
Affiliation:
Centre for Infection, Division of Clinical Sciences, St George's, University of London, Cranmer Terrace, London, United Kingdom. p0904768@sgul.ac.uk
Citation:
Characterisation of bovine leukocyte Ig-like receptors. 2012, 7 (4):e34291 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/246993
DOI:
10.1371/journal.pone.0034291
PubMed ID:
22485161
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHogan, Louiseen_GB
dc.contributor.authorBhuju, Sabinen_GB
dc.contributor.authorJones, Des Cen_GB
dc.contributor.authorLaing, Kenen_GB
dc.contributor.authorTrowsdale, Johnen_GB
dc.contributor.authorButcher, Philipen_GB
dc.contributor.authorSingh, Mahaviren_GB
dc.contributor.authorVordermeier, Martinen_GB
dc.contributor.authorAllen, Rachel Len_GB
dc.date.accessioned2012-10-04T11:26:46Z-
dc.date.available2012-10-04T11:26:46Z-
dc.date.issued2012-
dc.identifier.citationCharacterisation of bovine leukocyte Ig-like receptors. 2012, 7 (4):e34291 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22485161-
dc.identifier.doi10.1371/journal.pone.0034291-
dc.identifier.urihttp://hdl.handle.net/10033/246993-
dc.description.abstractLeukocyte Immunoglobulin-like receptors (LILR) are innate immune receptors involved in regulating both innate and adaptive immune functions. LILR show more interspecies conservation than the closely related Killer Ig-like receptors, and homologues have been identified in rodents, primates, seals and chickens. The murine equivalents, paired Ig-like receptors (PIR), contain two additional immunoglobulin domains, but show strong sequence and functional similarities to human LILR. The bovine genome was recently sequenced, with preliminary annotations indicating that LILR were present in this species. We therefore sought to identify and characterize novel LILR within the Bos taurus genome, compare these phylogenetically with LILR from other species and determine whether they were expressed in vivo. Twenty six potential bovine LILR were initially identified using BLAST and BLAT software. Phylogenetic analysis constructed using the neighbour-joining method, incorporating pairwise deletion and confidence limits estimated from 1000 replicates using bootstrapping, indicated that 16 of these represent novel bovine LILR. Protein structures defined using protein BLAST predict that the bovine LILR family comprises seven putative inhibitory, four activating and five soluble receptors. Preliminary expression analysis was performed by mapping the predicted sequences with raw data from total transcript sequence generated using Genome Analyzer IIx (Illumina) to provide evidence that all 16 of these receptors are expressed in vivo. The bovine receptor family appears to contain receptors which resemble the six domain rodent PIR as well as the four domain LILR found in other species.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshCattleen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshChromosome Mappingen_GB
dc.subject.meshGene Expressionen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLeukocytes, Mononuclearen_GB
dc.subject.meshMiceen_GB
dc.subject.meshModels, Molecularen_GB
dc.subject.meshPhylogenyen_GB
dc.subject.meshProtein Structure, Tertiaryen_GB
dc.subject.meshReceptors, Immunologicen_GB
dc.subject.meshSequence Analysis, Proteinen_GB
dc.subject.meshSequence Homology, Amino Aciden_GB
dc.titleCharacterisation of bovine leukocyte Ig-like receptors.en
dc.typeArticleen
dc.contributor.departmentCentre for Infection, Division of Clinical Sciences, St George's, University of London, Cranmer Terrace, London, United Kingdom. p0904768@sgul.ac.uken_GB
dc.identifier.journalPloS oneen_GB

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