Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells.

2.50
HDL Handle:
http://hdl.handle.net/10033/248355
Title:
Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells.
Authors:
Schallenberg, Sonja; Petzold, Cathleen; Tsai, Pei-Yun; Sparwasser, Tim; Kretschmer, Karsten
Abstract:
CD4(+)CD25(+) regulatory T (Treg) cell lineage commitment and expression of the transcription factor Foxp3 can be induced at the CD4(+)CD8(+) double-positive (DP) and CD4(+)CD8(?) single-positive stages of thymic development, as well as in postthymic CD4(+) T cells in peripheral lymphoid tissues. The availability of transgenic mice with Foxp3-dependent fluorochrome reporter gene expression has greatly facilitated studies on the intra- and extrathymic generation of murine Foxp3(+) Treg cells. Here, we performed a comparative analysis of thymic Treg cell development and peripheral compartments of mature Treg cells in various transgenic strains with gene targeted and bacterial artificial chromosome (BAC)-driven Foxp3-fluorochrome expression. These studies revealed a relative deficiency of Foxp3(+) DP thymocytes selectively in mice with targeted insertion of the fluorochrome reporter gene coding sequence into the endogenous Foxp3 gene. While Foxp3 BAC-driven fluorochrome expression in ex vivo CD4(+) T cells was found to faithfully reflect Foxp3 protein expression, we provide evidence that Foxp3 BAC transgenesis can result in sizable populations of Foxp3(+) Treg cells that lack fluorochrome reporter expression. This could be attributed to both timely delayed up-regulation of BAC expression in developing Treg cells and the accumulation of peripheral Foxp3(+) Treg cells with continuous transcriptional inactivity of the Foxp3 BAC transgene.
Affiliation:
Immunotolerance in Regeneration, CRTD/DFG-Center for Regenerative Therapies Dresden, Technical University Dresden, Dresden, Germany.
Citation:
Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells. 2012, 7 (8):e41971 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/248355
DOI:
10.1371/journal.pone.0041971
PubMed ID:
22879902
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSchallenberg, Sonjaen_GB
dc.contributor.authorPetzold, Cathleenen_GB
dc.contributor.authorTsai, Pei-Yunen_GB
dc.contributor.authorSparwasser, Timen_GB
dc.contributor.authorKretschmer, Karstenen_GB
dc.date.accessioned2012-10-11T10:41:14Z-
dc.date.available2012-10-11T10:41:14Z-
dc.date.issued2012-
dc.identifier.citationVagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells. 2012, 7 (8):e41971 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22879902-
dc.identifier.doi10.1371/journal.pone.0041971-
dc.identifier.urihttp://hdl.handle.net/10033/248355-
dc.description.abstractCD4(+)CD25(+) regulatory T (Treg) cell lineage commitment and expression of the transcription factor Foxp3 can be induced at the CD4(+)CD8(+) double-positive (DP) and CD4(+)CD8(?) single-positive stages of thymic development, as well as in postthymic CD4(+) T cells in peripheral lymphoid tissues. The availability of transgenic mice with Foxp3-dependent fluorochrome reporter gene expression has greatly facilitated studies on the intra- and extrathymic generation of murine Foxp3(+) Treg cells. Here, we performed a comparative analysis of thymic Treg cell development and peripheral compartments of mature Treg cells in various transgenic strains with gene targeted and bacterial artificial chromosome (BAC)-driven Foxp3-fluorochrome expression. These studies revealed a relative deficiency of Foxp3(+) DP thymocytes selectively in mice with targeted insertion of the fluorochrome reporter gene coding sequence into the endogenous Foxp3 gene. While Foxp3 BAC-driven fluorochrome expression in ex vivo CD4(+) T cells was found to faithfully reflect Foxp3 protein expression, we provide evidence that Foxp3 BAC transgenesis can result in sizable populations of Foxp3(+) Treg cells that lack fluorochrome reporter expression. This could be attributed to both timely delayed up-regulation of BAC expression in developing Treg cells and the accumulation of peripheral Foxp3(+) Treg cells with continuous transcriptional inactivity of the Foxp3 BAC transgene.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleVagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells.en
dc.typeArticleen
dc.contributor.departmentImmunotolerance in Regeneration, CRTD/DFG-Center for Regenerative Therapies Dresden, Technical University Dresden, Dresden, Germany.en_GB
dc.identifier.journalPloS oneen_GB

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