NKT cell stimulation with α-galactosylceramide results in a block of Th17 differentiation after intranasal immunization in mice.

2.50
Hdl Handle:
http://hdl.handle.net/10033/254172
Title:
NKT cell stimulation with α-galactosylceramide results in a block of Th17 differentiation after intranasal immunization in mice.
Authors:
Zygmunt, Beata M; Weissmann, Sebastian F; Guzman, Carlos A
Abstract:
In a previous study we demonstrated that intranasal (i.n.) vaccination promotes a Th17 biased immune response. Here, we show that co-administration of a pegylated derivative of α-galactosylceramide (αGCPEG) with an antigen, even in the presence of Th17-polarizing compounds, results in a strong blocking of Th17 differentiation. Additional studies demonstrated that this phenomenon is specifically dependent on soluble factors, like IL-4 and IFNγ, which are produced by NKT cells. Even NK1.1 negative NKT cells, which by themselves produce IL-17A, are able to block Th17 differentiation. It follows that the use of αGCPEG as adjuvant would enable to tailor Th17 responses, according to the specific clinical needs. This knowledge expands our understanding of the role played by NKT cells in overall control of the cytokine microenvironment, as well as in the overall shaping of adaptive immune responses.
Affiliation:
Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany. beata.zygmunt@bbsrc.ac.uk
Citation:
NKT cell stimulation with α-galactosylceramide results in a block of Th17 differentiation after intranasal immunization in mice. 2012, 7 (1):e30382 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/254172
DOI:
10.1371/journal.pone.0030382
PubMed ID:
22291945
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Vaccinology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorZygmunt, Beata Men_GB
dc.contributor.authorWeissmann, Sebastian Fen_GB
dc.contributor.authorGuzman, Carlos Aen_GB
dc.date.accessioned2012-11-30T20:32:40Z-
dc.date.available2012-11-30T20:32:40Z-
dc.date.issued2012-
dc.identifier.citationNKT cell stimulation with α-galactosylceramide results in a block of Th17 differentiation after intranasal immunization in mice. 2012, 7 (1):e30382 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22291945-
dc.identifier.doi10.1371/journal.pone.0030382-
dc.identifier.urihttp://hdl.handle.net/10033/254172-
dc.description.abstractIn a previous study we demonstrated that intranasal (i.n.) vaccination promotes a Th17 biased immune response. Here, we show that co-administration of a pegylated derivative of α-galactosylceramide (αGCPEG) with an antigen, even in the presence of Th17-polarizing compounds, results in a strong blocking of Th17 differentiation. Additional studies demonstrated that this phenomenon is specifically dependent on soluble factors, like IL-4 and IFNγ, which are produced by NKT cells. Even NK1.1 negative NKT cells, which by themselves produce IL-17A, are able to block Th17 differentiation. It follows that the use of αGCPEG as adjuvant would enable to tailor Th17 responses, according to the specific clinical needs. This knowledge expands our understanding of the role played by NKT cells in overall control of the cytokine microenvironment, as well as in the overall shaping of adaptive immune responses.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAdministration, Intranasalen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshCell Differentiationen_GB
dc.subject.meshCell Separationen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshDown-Regulationen_GB
dc.subject.meshGalactosylceramidesen_GB
dc.subject.meshImmunizationen_GB
dc.subject.meshLymphocyte Activationen_GB
dc.subject.meshMiceen_GB
dc.subject.meshMice, Inbred C57BLen_GB
dc.subject.meshMice, Knockouten_GB
dc.subject.meshNatural Killer T-Cellsen_GB
dc.subject.meshTh17 Cellsen_GB
dc.titleNKT cell stimulation with α-galactosylceramide results in a block of Th17 differentiation after intranasal immunization in mice.en
dc.typeArticleen
dc.contributor.departmentDepartment of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany. beata.zygmunt@bbsrc.ac.uken_GB
dc.identifier.journalPloS oneen_GB

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