Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/25908
Title:
Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation.
Authors:
Sztajer, Helena; Lemme, André; Vilchez, Ramiro; Schulz, Stefan; Geffers, Robert; Yip, Cindy Ying Yin; Levesque, Celine M; Cvitkovitch, Dennis G; Wagner-Döbler, Irene
Abstract:
Autoinducer 2 (AI-2) is the only species-nonspecific autoinducer known in bacteria and is produced by both gram-negative and gram-positive organisms. Consequently, it is proposed to function as a universal quorum-sensing signal for interaction between bacterial species. AI-2 is produced as the by-product of a metabolic transformation carried out by the LuxS enzyme. To separate the metabolic function of the LuxS enzyme from the signaling role of AI-2, we carried out a global transcriptome analysis of a luxS null mutant culture of Streptococcus mutans UA159, an important cariogenic bacterium and a crucial component of the dental plaque biofilm community, in comparison to a luxS null mutant culture supplemented with chemically pure 4,5-dihydroxy-2,3-pentanedione, the precursor of AI-2. The data revealed fundamental changes in gene expression affecting 585 genes (30% of the genome) which could not be restored by the signal molecule AI-2 and are therefore not caused by quorum sensing but by lack of the transformation carried out by the LuxS enzyme in the activated methyl cycle. All functional classes of enzymes were affected, including genes known to be important for biofilm formation, bacteriocin synthesis, competence, and acid tolerance. At the same time, 59 genes were identified whose transcription clearly responded to the addition of AI-2. Some of them were related to protein synthesis, stress, and cell division. Three membrane transport proteins were upregulated which are not related to any of the known AI-2 transporters. Three transcription factors were identified whose transcription was stimulated repeatedly by AI-2 addition during growth. Finally, a global regulatory protein, the delta subunit of the RNA polymerase (rpoE), was induced 147-fold by AI-2, representing the largest differential gene expression observed. The data show that many phenotypes related to the luxS mutation cannot be ascribed to quorum sensing and have identified for the first time regulatory proteins potentially mediating AI-2-based signaling in gram-positive bacteria.
Affiliation:
Helmholtz-Center for Infection Research, Division of Cell Biology, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation. 2008, 190 (1):401-15 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Jan-2008
URI:
http://hdl.handle.net/10033/25908
DOI:
10.1128/JB.01086-07
PubMed ID:
17981981
Type:
Article
Language:
en
ISSN:
1098-5530
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSztajer, Helena-
dc.contributor.authorLemme, André-
dc.contributor.authorVilchez, Ramiro-
dc.contributor.authorSchulz, Stefan-
dc.contributor.authorGeffers, Robert-
dc.contributor.authorYip, Cindy Ying Yin-
dc.contributor.authorLevesque, Celine M-
dc.contributor.authorCvitkovitch, Dennis G-
dc.contributor.authorWagner-Döbler, Irene-
dc.date.accessioned2008-05-14T10:24:21Z-
dc.date.available2008-05-14T10:24:21Z-
dc.date.issued2008-01-
dc.identifier.citationAutoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation. 2008, 190 (1):401-15 J. Bacteriol.en
dc.identifier.issn1098-5530-
dc.identifier.pmid17981981-
dc.identifier.doi10.1128/JB.01086-07-
dc.identifier.urihttp://hdl.handle.net/10033/25908-
dc.description.abstractAutoinducer 2 (AI-2) is the only species-nonspecific autoinducer known in bacteria and is produced by both gram-negative and gram-positive organisms. Consequently, it is proposed to function as a universal quorum-sensing signal for interaction between bacterial species. AI-2 is produced as the by-product of a metabolic transformation carried out by the LuxS enzyme. To separate the metabolic function of the LuxS enzyme from the signaling role of AI-2, we carried out a global transcriptome analysis of a luxS null mutant culture of Streptococcus mutans UA159, an important cariogenic bacterium and a crucial component of the dental plaque biofilm community, in comparison to a luxS null mutant culture supplemented with chemically pure 4,5-dihydroxy-2,3-pentanedione, the precursor of AI-2. The data revealed fundamental changes in gene expression affecting 585 genes (30% of the genome) which could not be restored by the signal molecule AI-2 and are therefore not caused by quorum sensing but by lack of the transformation carried out by the LuxS enzyme in the activated methyl cycle. All functional classes of enzymes were affected, including genes known to be important for biofilm formation, bacteriocin synthesis, competence, and acid tolerance. At the same time, 59 genes were identified whose transcription clearly responded to the addition of AI-2. Some of them were related to protein synthesis, stress, and cell division. Three membrane transport proteins were upregulated which are not related to any of the known AI-2 transporters. Three transcription factors were identified whose transcription was stimulated repeatedly by AI-2 addition during growth. Finally, a global regulatory protein, the delta subunit of the RNA polymerase (rpoE), was induced 147-fold by AI-2, representing the largest differential gene expression observed. The data show that many phenotypes related to the luxS mutation cannot be ascribed to quorum sensing and have identified for the first time regulatory proteins potentially mediating AI-2-based signaling in gram-positive bacteria.en
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshCarbon-Sulfur Lyasesen
dc.subject.meshDNA Primersen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Complementaryen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenome, Bacterialen
dc.subject.meshHomoserineen
dc.subject.meshLactonesen
dc.subject.meshMutationen
dc.subject.meshNucleic Acid Hybridizationen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshQuorum Sensingen
dc.subject.meshRNA, Bacterialen
dc.subject.meshStreptococcus mutansen
dc.titleAutoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Center for Infection Research, Division of Cell Biology, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalJournal of bacteriologyen

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