2.50
Hdl Handle:
http://hdl.handle.net/10033/265513
Title:
Specific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus.
Authors:
Ejaz, Asim; Steinmann, Eike; Bánki, Zoltán; Anggakusuma; Khalid, Sana; Lengauer, Susanne; Wilhelm, Corinne; Zoller, Heinz; Schloegl, Anna; Steinmann, Joerg; Grabski, Elena; Kleines, Michael; Pietschmann, Thomas; Stoiber, Heribert
Abstract:
Viruses of different families encode for regulators of the complement system (RCAs) or acquire such RCAs from the host to get protection against complement-mediated lysis (CML). As hepatitis C virus (HCV) shares no genetic similarity to any known RCA and is detectable at high titers in sera of infected individuals, we investigated whether HCV has adapted host-derived RCAs to resist CML. Here we report that HCV selectively incorporates CD59 while neither CD55, nor CD46 are associated with the virus. The presence of CD59 was shown by capture assays using patient- and cell culture-derived HCV isolates. Association of CD59 with HCV was further confirmed by Western blot analysis using purified viral supernatants from infected Huh 7.5 cells. HCV captured by antibodies specific for CD59 remained infectious for Huh 7.5 cells. In addition, blocking of CD59 in the presence of active complement reduced the titer of HCV most likely due to CML. HCV produced in CD59 knock-down cells were more significantly susceptible to CML compared to wild type virus, but neither replication, assembly nor infectivity of the virus seemed to be impaired in the absence of CD59. In summary our data indicate that HCV incorporates selectively CD59 in its envelope to gain resistance to CML in serum of infected individuals.
Affiliation:
Institute of Virology, Innsbruck Medical University, Innsbruck, Austria.
Citation:
Specific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus. 2012, 7 (9):e45770 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/265513
DOI:
10.1371/journal.pone.0045770
PubMed ID:
23049856
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorEjaz, Asimen_GB
dc.contributor.authorSteinmann, Eikeen_GB
dc.contributor.authorBánki, Zoltánen_GB
dc.contributor.authorAnggakusumaen_GB
dc.contributor.authorKhalid, Sanaen_GB
dc.contributor.authorLengauer, Susanneen_GB
dc.contributor.authorWilhelm, Corinneen_GB
dc.contributor.authorZoller, Heinzen_GB
dc.contributor.authorSchloegl, Annaen_GB
dc.contributor.authorSteinmann, Joergen_GB
dc.contributor.authorGrabski, Elenaen_GB
dc.contributor.authorKleines, Michaelen_GB
dc.contributor.authorPietschmann, Thomasen_GB
dc.contributor.authorStoiber, Heriberten_GB
dc.date.accessioned2013-01-15T15:33:08Z-
dc.date.available2013-01-15T15:33:08Z-
dc.date.issued2012-
dc.identifier.citationSpecific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus. 2012, 7 (9):e45770 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid23049856-
dc.identifier.doi10.1371/journal.pone.0045770-
dc.identifier.urihttp://hdl.handle.net/10033/265513-
dc.description.abstractViruses of different families encode for regulators of the complement system (RCAs) or acquire such RCAs from the host to get protection against complement-mediated lysis (CML). As hepatitis C virus (HCV) shares no genetic similarity to any known RCA and is detectable at high titers in sera of infected individuals, we investigated whether HCV has adapted host-derived RCAs to resist CML. Here we report that HCV selectively incorporates CD59 while neither CD55, nor CD46 are associated with the virus. The presence of CD59 was shown by capture assays using patient- and cell culture-derived HCV isolates. Association of CD59 with HCV was further confirmed by Western blot analysis using purified viral supernatants from infected Huh 7.5 cells. HCV captured by antibodies specific for CD59 remained infectious for Huh 7.5 cells. In addition, blocking of CD59 in the presence of active complement reduced the titer of HCV most likely due to CML. HCV produced in CD59 knock-down cells were more significantly susceptible to CML compared to wild type virus, but neither replication, assembly nor infectivity of the virus seemed to be impaired in the absence of CD59. In summary our data indicate that HCV incorporates selectively CD59 in its envelope to gain resistance to CML in serum of infected individuals.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleSpecific acquisition of functional CD59 but not CD46 or CD55 by hepatitis C virus.en
dc.typeArticleen
dc.contributor.departmentInstitute of Virology, Innsbruck Medical University, Innsbruck, Austria.en_GB
dc.identifier.journalPloS oneen_GB

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