Variability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine.

2.50
Hdl Handle:
http://hdl.handle.net/10033/266617
Title:
Variability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine.
Authors:
Sagar, Vivek; Bergmann, René; Nerlich, Andreas; McMillan, David J; Nitsche Schmitz, D Patric; Chhatwal, Gursharan S
Abstract:
Streptococcus pyogenes causes a wide variety of human diseases and is a significant cause of morbidity and mortality. Attempts to develop a vaccine were hampered by the genetic diversity of S. pyogenes across different regions of the world. This study sought to identify streptococcal antigens suitable for a region-specific vaccine in India. We used a two-step approach, first performing epidemiological analysis to identify the conserved antigens among Indian isolates. The second step consisted of validating the identified antigens by serological analysis. The 201 streptococcal clinical isolates from India used in this study represented 69 different emm types, with emm12 being the most prevalent. Virulence profiling of the North and South Indian S. pyogenes isolates with a custom-designed streptococcal virulence microarray identified seven conserved putative vaccine candidates. Collagen-like surface protein (SCI), putative secreted 5'-nucleotidase (PSNT), and C5a peptidase were found in 100% of the isolates, while R28, a putative surface antigen (PSA), and a hypothetical protein (HYP) were found in 90% of the isolates. A fibronectin binding protein, SfbI, was present in only 78% of the isolates. In order to validate the identified potential vaccine candidates, 185 serum samples obtained from patients with different clinical manifestations were tested for antibodies. Irrespective of clinical manifestations, serum samples showed high antibody titers to all proteins except for SCI and R28. Thus, the data indicate that PSNT, C5a peptidase, PSA, HYP, and SfbI are promising candidates for a region-specific streptococcal vaccine for the different parts of India.
Affiliation:
Department of Medical Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Variability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine. 2012, 19 (11):1818-25 Clin. Vaccine Immunol.
Journal:
Clinical and vaccine immunology : CVI
Issue Date:
Nov-2012
URI:
http://hdl.handle.net/10033/266617
DOI:
10.1128/CVI.00112-12
PubMed ID:
22971782
Type:
Article
Language:
en
ISSN:
1556-679X
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorSagar, Viveken_GB
dc.contributor.authorBergmann, Renéen_GB
dc.contributor.authorNerlich, Andreasen_GB
dc.contributor.authorMcMillan, David Jen_GB
dc.contributor.authorNitsche Schmitz, D Patricen_GB
dc.contributor.authorChhatwal, Gursharan Sen_GB
dc.date.accessioned2013-01-23T10:13:11Z-
dc.date.available2013-01-23T10:13:11Z-
dc.date.issued2012-11-
dc.identifier.citationVariability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine. 2012, 19 (11):1818-25 Clin. Vaccine Immunol.en_GB
dc.identifier.issn1556-679X-
dc.identifier.pmid22971782-
dc.identifier.doi10.1128/CVI.00112-12-
dc.identifier.urihttp://hdl.handle.net/10033/266617-
dc.description.abstractStreptococcus pyogenes causes a wide variety of human diseases and is a significant cause of morbidity and mortality. Attempts to develop a vaccine were hampered by the genetic diversity of S. pyogenes across different regions of the world. This study sought to identify streptococcal antigens suitable for a region-specific vaccine in India. We used a two-step approach, first performing epidemiological analysis to identify the conserved antigens among Indian isolates. The second step consisted of validating the identified antigens by serological analysis. The 201 streptococcal clinical isolates from India used in this study represented 69 different emm types, with emm12 being the most prevalent. Virulence profiling of the North and South Indian S. pyogenes isolates with a custom-designed streptococcal virulence microarray identified seven conserved putative vaccine candidates. Collagen-like surface protein (SCI), putative secreted 5'-nucleotidase (PSNT), and C5a peptidase were found in 100% of the isolates, while R28, a putative surface antigen (PSA), and a hypothetical protein (HYP) were found in 90% of the isolates. A fibronectin binding protein, SfbI, was present in only 78% of the isolates. In order to validate the identified potential vaccine candidates, 185 serum samples obtained from patients with different clinical manifestations were tested for antibodies. Irrespective of clinical manifestations, serum samples showed high antibody titers to all proteins except for SCI and R28. Thus, the data indicate that PSNT, C5a peptidase, PSA, HYP, and SfbI are promising candidates for a region-specific streptococcal vaccine for the different parts of India.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Clinical and vaccine immunology : CVIen_GB
dc.titleVariability in the distribution of genes encoding virulence factors and putative extracellular proteins of Streptococcus pyogenes in India, a region with high streptococcal disease burden, and implication for development of a regional multisubunit vaccine.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.en_GB
dc.identifier.journalClinical and vaccine immunology : CVIen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.