Crp Induces Switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and Links Nutritional Status to Virulence.

2.50
Hdl Handle:
http://hdl.handle.net/10033/269594
Title:
Crp Induces Switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and Links Nutritional Status to Virulence.
Authors:
Heroven, Ann Kathrin; Sest, Maike; Pisano, Fabio; Scheb-Wetzel, Matthias; Steinmann, Rebekka; Böhme, Katja; Klein, Johannes; Münch, Richard; Schomburg, Dietmar; Dersch, Petra
Abstract:
Colonization of the intestinal tract and dissemination into deeper tissues by the enteric pathogen Yersinia pseudotuberculosis demands expression of a special set of virulence factors important for the initiation and the persistence of the infection. In this study we demonstrate that many virulence-associated functions are coregulated with the carbohydrate metabolism. This link is mediated by the carbon storage regulator (Csr) system, including the regulatory RNAs CsrB and CsrC, and the cAMP receptor protein (Crp), which both control virulence gene expression in response to the nutrient composition of the medium. Here, we show that Crp regulates the synthesis of both Csr RNAs in an opposite manner. A loss of the crp gene resulted in a strong upregulation of CsrB synthesis, whereas CsrC levels were strongly reduced leading to downregulation of the virulence regulator RovA. Switching of the Csr RNA involves Crp-mediated repression of the response regulator UvrY which activates csrB transcription. To elucidate the regulatory links between virulence and carbon metabolism, we performed comparative metabolome, transcriptome, and phenotypic microarray analyses and found that Crp promotes oxidative catabolism of many different carbon sources, whereas fermentative patterns of metabolism are favored when crp is deleted. Mouse infection experiments further demonstrated that Crp is pivotal for a successful Y. pseudotuberculosis infection. In summary, placement of the Csr system and important virulence factors under control of Crp enables this pathogen to link its nutritional status to virulence in order to optimize biological fitness and infection efficiency through the infectious life cycle.
Affiliation:
Abteilung Molekulare Infektionsbiologie, Helmholtz-Zentrum für Infektionsforschung Braunschweig, Germany.
Citation:
Crp Induces Switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and Links Nutritional Status to Virulence. 2012, 2:158 Front Cell Infect Microbiol
Journal:
Frontiers in cellular and infection microbiology
Issue Date:
2012
URI:
http://hdl.handle.net/10033/269594
DOI:
10.3389/fcimb.2012.00158
PubMed ID:
23251905
Type:
Article
Language:
en
ISSN:
2235-2988
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorHeroven, Ann Kathrinen_GB
dc.contributor.authorSest, Maikeen_GB
dc.contributor.authorPisano, Fabioen_GB
dc.contributor.authorScheb-Wetzel, Matthiasen_GB
dc.contributor.authorSteinmann, Rebekkaen_GB
dc.contributor.authorBöhme, Katjaen_GB
dc.contributor.authorKlein, Johannesen_GB
dc.contributor.authorMünch, Richarden_GB
dc.contributor.authorSchomburg, Dietmaren_GB
dc.contributor.authorDersch, Petraen_GB
dc.date.accessioned2013-02-14T15:55:40Z-
dc.date.available2013-02-14T15:55:40Z-
dc.date.issued2012-
dc.identifier.citationCrp Induces Switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and Links Nutritional Status to Virulence. 2012, 2:158 Front Cell Infect Microbiolen_GB
dc.identifier.issn2235-2988-
dc.identifier.pmid23251905-
dc.identifier.doi10.3389/fcimb.2012.00158-
dc.identifier.urihttp://hdl.handle.net/10033/269594-
dc.description.abstractColonization of the intestinal tract and dissemination into deeper tissues by the enteric pathogen Yersinia pseudotuberculosis demands expression of a special set of virulence factors important for the initiation and the persistence of the infection. In this study we demonstrate that many virulence-associated functions are coregulated with the carbohydrate metabolism. This link is mediated by the carbon storage regulator (Csr) system, including the regulatory RNAs CsrB and CsrC, and the cAMP receptor protein (Crp), which both control virulence gene expression in response to the nutrient composition of the medium. Here, we show that Crp regulates the synthesis of both Csr RNAs in an opposite manner. A loss of the crp gene resulted in a strong upregulation of CsrB synthesis, whereas CsrC levels were strongly reduced leading to downregulation of the virulence regulator RovA. Switching of the Csr RNA involves Crp-mediated repression of the response regulator UvrY which activates csrB transcription. To elucidate the regulatory links between virulence and carbon metabolism, we performed comparative metabolome, transcriptome, and phenotypic microarray analyses and found that Crp promotes oxidative catabolism of many different carbon sources, whereas fermentative patterns of metabolism are favored when crp is deleted. Mouse infection experiments further demonstrated that Crp is pivotal for a successful Y. pseudotuberculosis infection. In summary, placement of the Csr system and important virulence factors under control of Crp enables this pathogen to link its nutritional status to virulence in order to optimize biological fitness and infection efficiency through the infectious life cycle.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Frontiers in cellular and infection microbiologyen_GB
dc.titleCrp Induces Switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and Links Nutritional Status to Virulence.en
dc.typeArticleen
dc.contributor.departmentAbteilung Molekulare Infektionsbiologie, Helmholtz-Zentrum für Infektionsforschung Braunschweig, Germany.en_GB
dc.identifier.journalFrontiers in cellular and infection microbiologyen_GB

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