Impact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing.

2.50
Hdl Handle:
http://hdl.handle.net/10033/270375
Title:
Impact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing.
Authors:
Hamisch, Domenica; Randewig, Dörte; Schliesky, Simon; Bräutigam, Andrea; Weber, Andreas P M; Geffers, Robert; Herschbach, Cornelia; Rennenberg, Heinz; Mendel, Ralf R; Hänsch, Robert
Abstract:
High concentrations of sulfur dioxide (SO(2) ) as an air pollutant, and its derivative sulfite, cause abiotic stress that can lead to cell death. It is currently unknown to what extent plant fumigation triggers specific transcriptional responses. To address this question, and to test the hypothesis that sulfite oxidase (SO) is acting in SO(2) detoxification, we compared Arabidopsis wildtype (WT) and SO knockout lines (SO-KO) facing the impact of 600 nl l(-1) SO(2) , using RNAseq to quantify absolute transcript abundances. These transcriptome data were correlated to sulfur metabolism-related enzyme activities and metabolites obtained from identical samples in a previous study. SO-KO plants exhibited remarkable and broad regulative responses at the mRNA level, especially in transcripts related to sulfur metabolism enzymes, but also in those related to stress response and senescence. Focusing on SO regulation, no alterations were detectable in the WT, whereas in SO-KO plants we found up-regulation of two splice variants of the SO gene, although this gene is not functional in this line. Our data provide evidence for the highly specific coregulation between SO and sulfur-related enzymes like APS reductase, and suggest two novel candidates for involvement in SO(2) detoxification: an apoplastic peroxidase, and defensins as putative cysteine mass storages.
Affiliation:
Institut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.
Citation:
Impact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing. 2012, 196 (4):1074-85 New Phytol.
Journal:
The New phytologist
Issue Date:
Dec-2012
URI:
http://hdl.handle.net/10033/270375
DOI:
10.1111/j.1469-8137.2012.04331.x
PubMed ID:
23025405
Type:
Article
Language:
en
ISSN:
1469-8137
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorHamisch, Domenicaen_GB
dc.contributor.authorRandewig, Dörteen_GB
dc.contributor.authorSchliesky, Simonen_GB
dc.contributor.authorBräutigam, Andreaen_GB
dc.contributor.authorWeber, Andreas P Men_GB
dc.contributor.authorGeffers, Roberten_GB
dc.contributor.authorHerschbach, Corneliaen_GB
dc.contributor.authorRennenberg, Heinzen_GB
dc.contributor.authorMendel, Ralf Ren_GB
dc.contributor.authorHänsch, Roberten_GB
dc.date.accessioned2013-02-25T14:46:34Z-
dc.date.available2013-02-25T14:46:34Z-
dc.date.issued2012-12-
dc.identifier.citationImpact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing. 2012, 196 (4):1074-85 New Phytol.en_GB
dc.identifier.issn1469-8137-
dc.identifier.pmid23025405-
dc.identifier.doi10.1111/j.1469-8137.2012.04331.x-
dc.identifier.urihttp://hdl.handle.net/10033/270375-
dc.description.abstractHigh concentrations of sulfur dioxide (SO(2) ) as an air pollutant, and its derivative sulfite, cause abiotic stress that can lead to cell death. It is currently unknown to what extent plant fumigation triggers specific transcriptional responses. To address this question, and to test the hypothesis that sulfite oxidase (SO) is acting in SO(2) detoxification, we compared Arabidopsis wildtype (WT) and SO knockout lines (SO-KO) facing the impact of 600 nl l(-1) SO(2) , using RNAseq to quantify absolute transcript abundances. These transcriptome data were correlated to sulfur metabolism-related enzyme activities and metabolites obtained from identical samples in a previous study. SO-KO plants exhibited remarkable and broad regulative responses at the mRNA level, especially in transcripts related to sulfur metabolism enzymes, but also in those related to stress response and senescence. Focusing on SO regulation, no alterations were detectable in the WT, whereas in SO-KO plants we found up-regulation of two splice variants of the SO gene, although this gene is not functional in this line. Our data provide evidence for the highly specific coregulation between SO and sulfur-related enzymes like APS reductase, and suggest two novel candidates for involvement in SO(2) detoxification: an apoplastic peroxidase, and defensins as putative cysteine mass storages.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The New phytologisten_GB
dc.titleImpact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing.en
dc.typeArticleen
dc.contributor.departmentInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.en_GB
dc.identifier.journalThe New phytologisten_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.