A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10033/271194
Title:
A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo.
Authors:
Onder, Lucas; Scandella, Elke; Chai, Qian; Firner, Sonja; Mayer, Christian T; Sparwasser, Tim ( 0000-0001-5645-902X ) ; Thiel, Volker; Rülicke, Thomas; Ludewig, Burkhard
Abstract:
Stromal cells provide the structural foundation of secondary lymphoid organs (SLOs), and regulate leukocyte access and cell migration within the different compartments of spleen and lymph nodes (LNs). Furthermore, several stromal cell subsets have been implied in shaping of T cell responses through direct presentation of antigen. Despite significant gain of knowledge on the biology of different SLO-resident stromal cell subsets, their molecular and functional characterization has remained incomplete. To address this need, we have generated a bacterial artificial chromosome-transgenic mouse model that utilizes the podoplanin (pdpn) promoter to express the Cre-recombinase exclusively in stromal cells of SLOs. The characterization of the Pdpn-Cre mouse revealed transgene expression in subsets of fibroblastic reticular cells and lymphatic endothelial cells in LNs. Furthermore, the transgene facilitated the identification of a novel splenic perivascular stromal cell subpopulation that forms web-like structures around central arterioles. Assessment of the in vivo antigen expression in the genetically tagged stromal cells in Pdpn-Cre mice revealed activation of both MHC I and II-restricted TCR transgenic T cells. Taken together, stromal pdpn-Cre expression is well-suited to characterize the phenotype and to dissect the function of lymphoid organ stromal cells.
Affiliation:
Institute of Immunobiology, Cantonal Hospital St. Gallen St. Gallen, Switzerland.
Citation:
A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo. 2011, 2:50 Front Immunol
Journal:
Frontiers in immunology
Issue Date:
2011
URI:
http://hdl.handle.net/10033/271194
DOI:
10.3389/fimmu.2011.00050
PubMed ID:
22566840
Type:
Article
Language:
en
ISSN:
1664-3224
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValue Language
dc.contributor.authorOnder, Lucasen_GB
dc.contributor.authorScandella, Elkeen_GB
dc.contributor.authorChai, Qianen_GB
dc.contributor.authorFirner, Sonjaen_GB
dc.contributor.authorMayer, Christian Ten_GB
dc.contributor.authorSparwasser, Timen_GB
dc.contributor.authorThiel, Volkeren_GB
dc.contributor.authorRülicke, Thomasen_GB
dc.contributor.authorLudewig, Burkharden_GB
dc.date.accessioned2013-03-05T15:15:47Zen
dc.date.available2013-03-05T15:15:47Zen
dc.date.issued2011en
dc.identifier.citationA novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo. 2011, 2:50 Front Immunolen_GB
dc.identifier.issn1664-3224en
dc.identifier.pmid22566840en
dc.identifier.doi10.3389/fimmu.2011.00050en
dc.identifier.urihttp://hdl.handle.net/10033/271194en
dc.description.abstractStromal cells provide the structural foundation of secondary lymphoid organs (SLOs), and regulate leukocyte access and cell migration within the different compartments of spleen and lymph nodes (LNs). Furthermore, several stromal cell subsets have been implied in shaping of T cell responses through direct presentation of antigen. Despite significant gain of knowledge on the biology of different SLO-resident stromal cell subsets, their molecular and functional characterization has remained incomplete. To address this need, we have generated a bacterial artificial chromosome-transgenic mouse model that utilizes the podoplanin (pdpn) promoter to express the Cre-recombinase exclusively in stromal cells of SLOs. The characterization of the Pdpn-Cre mouse revealed transgene expression in subsets of fibroblastic reticular cells and lymphatic endothelial cells in LNs. Furthermore, the transgene facilitated the identification of a novel splenic perivascular stromal cell subpopulation that forms web-like structures around central arterioles. Assessment of the in vivo antigen expression in the genetically tagged stromal cells in Pdpn-Cre mice revealed activation of both MHC I and II-restricted TCR transgenic T cells. Taken together, stromal pdpn-Cre expression is well-suited to characterize the phenotype and to dissect the function of lymphoid organ stromal cells.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Frontiers in immunologyen_GB
dc.titleA novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo.en
dc.typeArticleen
dc.contributor.departmentInstitute of Immunobiology, Cantonal Hospital St. Gallen St. Gallen, Switzerland.en_GB
dc.identifier.journalFrontiers in immunologyen_GB
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