The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/281672
Title:
The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation.
Authors:
Haenebalcke, Lieven; Goossens, Steven; Dierickx, Pieterjan; Bartunkova, Sonia; D'Hont, Jinke; Haigh, Katharina; Hochepied, Tino; Wirth, Dagmar; Nagy, Andras; Haigh, Jody J
Abstract:
Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.
Affiliation:
Vascular Cell Biology Unit, VIB Department for Molecular Biomedical Research, Ghent University, Technologiepark 927, 9052 Zwijnaarde Ghent, Belgium.
Citation:
The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation. 2013, 3 (2):335-41 Cell Rep
Journal:
Cell reports
Issue Date:
21-Feb-2013
URI:
http://hdl.handle.net/10033/281672
DOI:
10.1016/j.celrep.2013.01.016
PubMed ID:
23395636
Type:
Article
Language:
en
ISSN:
2211-1247
Appears in Collections:
publications of the research group modell systems for infections and immunity (MSYS)

Full metadata record

DC FieldValue Language
dc.contributor.authorHaenebalcke, Lievenen_GB
dc.contributor.authorGoossens, Stevenen_GB
dc.contributor.authorDierickx, Pieterjanen_GB
dc.contributor.authorBartunkova, Soniaen_GB
dc.contributor.authorD'Hont, Jinkeen_GB
dc.contributor.authorHaigh, Katharinaen_GB
dc.contributor.authorHochepied, Tinoen_GB
dc.contributor.authorWirth, Dagmaren_GB
dc.contributor.authorNagy, Andrasen_GB
dc.contributor.authorHaigh, Jody Jen_GB
dc.date.accessioned2013-04-17T14:22:19Z-
dc.date.available2013-04-17T14:22:19Z-
dc.date.issued2013-02-21-
dc.identifier.citationThe ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation. 2013, 3 (2):335-41 Cell Repen_GB
dc.identifier.issn2211-1247-
dc.identifier.pmid23395636-
dc.identifier.doi10.1016/j.celrep.2013.01.016-
dc.identifier.urihttp://hdl.handle.net/10033/281672-
dc.description.abstractControl of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Cell reportsen_GB
dc.titleThe ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation.en
dc.typeArticleen
dc.contributor.departmentVascular Cell Biology Unit, VIB Department for Molecular Biomedical Research, Ghent University, Technologiepark 927, 9052 Zwijnaarde Ghent, Belgium.en_GB
dc.identifier.journalCell reportsen_GB

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