Development and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology.

2.50
Hdl Handle:
http://hdl.handle.net/10033/283779
Title:
Development and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology.
Authors:
Yevsa, Tetyana; Ebensen, Thomas ( 0000-0001-8906-063X ) ; Fuchs, Barbara; Zygmunt, Beata; Libanova, Rimma; Gross, Roy; Schulze, Kai ( 0000-0003-2286-3416 ) ; Guzmán, Carlos A
Abstract:
Bordetella bronchiseptica is an important pathogen causing a number of veterinary respiratory syndromes in agriculturally important and food-producing confinement-reared animals, resulting in great economic losses annually amounting to billions of euros worldwide. Currently available live vaccines are incompletely satisfactory in terms of efficacy and safety. An efficient vaccine for livestock animals would allow reducing the application of antibiotics, thereby preventing the massive release of pharmaceuticals into the environment. Here, we describe two new potential vaccine strains based on the BB7865 strain. Two independent attenuating mutations were incorporated by homologous recombination in order to make negligible the risk of recombination and subsequent reversion to the virulent phenotype. The mutations are critical for bacterial metabolism, resistance to oxidative stress, intracellular survival and in vivo persistence. The resulting double mutants BB7865 risA aroA and BB7865 risA dapE were characterized as promising vaccine candidates, which are able to confer protection against colonization of the lower respiratory tract after sublethal challenge with the wild-type strain.
Affiliation:
Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, D-38124 Braunschweig, Germany.
Citation:
Development and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology. 2013, 15 (1):64-76 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10033/283779
DOI:
10.1111/j.1462-2920.2012.02779.x
PubMed ID:
22676396
Type:
Article
Language:
en
ISSN:
1462-2920
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorYevsa, Tetyanaen_GB
dc.contributor.authorEbensen, Thomasen_GB
dc.contributor.authorFuchs, Barbaraen_GB
dc.contributor.authorZygmunt, Beataen_GB
dc.contributor.authorLibanova, Rimmaen_GB
dc.contributor.authorGross, Royen_GB
dc.contributor.authorSchulze, Kaien_GB
dc.contributor.authorGuzmán, Carlos Aen_GB
dc.date.accessioned2013-04-24T14:32:39Zen
dc.date.available2013-04-24T14:32:39Zen
dc.date.issued2013-01en
dc.identifier.citationDevelopment and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology. 2013, 15 (1):64-76 Environ. Microbiol.en_GB
dc.identifier.issn1462-2920en
dc.identifier.pmid22676396en
dc.identifier.doi10.1111/j.1462-2920.2012.02779.xen
dc.identifier.urihttp://hdl.handle.net/10033/283779en
dc.description.abstractBordetella bronchiseptica is an important pathogen causing a number of veterinary respiratory syndromes in agriculturally important and food-producing confinement-reared animals, resulting in great economic losses annually amounting to billions of euros worldwide. Currently available live vaccines are incompletely satisfactory in terms of efficacy and safety. An efficient vaccine for livestock animals would allow reducing the application of antibiotics, thereby preventing the massive release of pharmaceuticals into the environment. Here, we describe two new potential vaccine strains based on the BB7865 strain. Two independent attenuating mutations were incorporated by homologous recombination in order to make negligible the risk of recombination and subsequent reversion to the virulent phenotype. The mutations are critical for bacterial metabolism, resistance to oxidative stress, intracellular survival and in vivo persistence. The resulting double mutants BB7865 risA aroA and BB7865 risA dapE were characterized as promising vaccine candidates, which are able to confer protection against colonization of the lower respiratory tract after sublethal challenge with the wild-type strain.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Environmental microbiologyen_GB
dc.titleDevelopment and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology.en
dc.typeArticleen
dc.contributor.departmentDepartment of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, D-38124 Braunschweig, Germany.en_GB
dc.identifier.journalEnvironmental microbiologyen_GB

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