Three stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water.

2.50
Hdl Handle:
http://hdl.handle.net/10033/294328
Title:
Three stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water.
Authors:
Macedo, Alexandre José; Kuhlicke, Ute; Neu, Thomas R; Timmis, Kenneth N; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 )
Abstract:
Soil contaminated with polychlorinated biphenyls (PCB) was used as an inoculum to grow a complex biofilm community on PCB oil (Aroclor 1242) on a substratum (Permanox). The biofilm was monitored for 31 days by confocal laser scanning microscopy, community fingerprinting using single-strand conformational polymorphism (SSCP), amplicons of the 16S rRNA genes, and chemical analyses of the PCB congeners. SSCP analysis of the young biofilm revealed a rather diverse microbial community with species of the genera Herbaspirillum and Bradyrhizobium as dominant members. The biofilm developing on the PCB droplets displayed pronounced stages of PCB degradation and biofilm development not described before from pure-culture experiments. The first step was the colonization of the substratum while the PCB oil was hardly populated. When a certain density of bacteria was reached on the Permanox, the PCB was colonized, but soon the degradation of the congeners was markedly reduced and many cells were damaged, as seen by LIVE/DEAD staining. Finally, the biofilm formed aggregates and invaded the PCB oil, showing lower numbers of damaged cells than before and a dramatic increase in PCB degradation. This sequence of biofilm formation is understood as a maturation process prior to PCB oil colonization. This is followed by a thin biofilm on the PCB droplet, an aggregation process forming pockets in the PCB, and finally an invasion of the biofilm into the PCB oil. Only the mature biofilm showed degradation of pentachlorinated PCB congeners, which may be reductively dechlorinated and the resulting trichlorobiphenyls then aerobically metabolized.
Affiliation:
GBF-National Research Center for Biotechnology, Environmental Microbiology, Mascheroder Weg 1, 38124 Braunschweig, Germany.
Citation:
Three stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water. 2005, 71 (11):7301-9 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
Nov-2005
URI:
http://hdl.handle.net/10033/294328
DOI:
10.1128/AEM.71.11.7301-7309.2005
PubMed ID:
16269772
Type:
Article
Language:
en
ISSN:
0099-2240
Appears in Collections:
publications of the research group chemical microbiology (CMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorMacedo, Alexandre Joséen_GB
dc.contributor.authorKuhlicke, Uteen_GB
dc.contributor.authorNeu, Thomas Ren_GB
dc.contributor.authorTimmis, Kenneth Nen_GB
dc.contributor.authorAbraham, Wolf-Raineren_GB
dc.date.accessioned2013-06-21T12:06:07Zen
dc.date.available2013-06-21T12:06:07Zen
dc.date.issued2005-11en
dc.identifier.citationThree stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water. 2005, 71 (11):7301-9 Appl. Environ. Microbiol.en_GB
dc.identifier.issn0099-2240en
dc.identifier.pmid16269772en
dc.identifier.doi10.1128/AEM.71.11.7301-7309.2005en
dc.identifier.urihttp://hdl.handle.net/10033/294328en
dc.description.abstractSoil contaminated with polychlorinated biphenyls (PCB) was used as an inoculum to grow a complex biofilm community on PCB oil (Aroclor 1242) on a substratum (Permanox). The biofilm was monitored for 31 days by confocal laser scanning microscopy, community fingerprinting using single-strand conformational polymorphism (SSCP), amplicons of the 16S rRNA genes, and chemical analyses of the PCB congeners. SSCP analysis of the young biofilm revealed a rather diverse microbial community with species of the genera Herbaspirillum and Bradyrhizobium as dominant members. The biofilm developing on the PCB droplets displayed pronounced stages of PCB degradation and biofilm development not described before from pure-culture experiments. The first step was the colonization of the substratum while the PCB oil was hardly populated. When a certain density of bacteria was reached on the Permanox, the PCB was colonized, but soon the degradation of the congeners was markedly reduced and many cells were damaged, as seen by LIVE/DEAD staining. Finally, the biofilm formed aggregates and invaded the PCB oil, showing lower numbers of damaged cells than before and a dramatic increase in PCB degradation. This sequence of biofilm formation is understood as a maturation process prior to PCB oil colonization. This is followed by a thin biofilm on the PCB droplet, an aggregation process forming pockets in the PCB, and finally an invasion of the biofilm into the PCB oil. Only the mature biofilm showed degradation of pentachlorinated PCB congeners, which may be reductively dechlorinated and the resulting trichlorobiphenyls then aerobically metabolized.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Applied and environmental microbiologyen_GB
dc.subject.meshBacteriaen_GB
dc.subject.meshBiodegradation, Environmentalen_GB
dc.subject.meshBiofilmsen_GB
dc.subject.meshBradyrhizobiumen_GB
dc.subject.meshEcosystemen_GB
dc.subject.meshHerbaspirillumen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshPolychlorinated Biphenylsen_GB
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_GB
dc.subject.meshRNA, Ribosomal, 16Sen_GB
dc.subject.meshSequence Analysis, DNAen_GB
dc.subject.meshSoil Microbiologyen_GB
dc.subject.meshSoil Pollutantsen_GB
dc.subject.meshTime Factorsen_GB
dc.subject.meshWateren_GB
dc.titleThree stages of a biofilm community developing at the liquid-liquid interface between polychlorinated biphenyls and water.en
dc.typeArticleen
dc.contributor.departmentGBF-National Research Center for Biotechnology, Environmental Microbiology, Mascheroder Weg 1, 38124 Braunschweig, Germany.en_GB
dc.identifier.journalApplied and environmental microbiologyen_GB

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