2.50
Hdl Handle:
http://hdl.handle.net/10033/295974
Title:
Cell culture systems for hepatitis C virus.
Authors:
Steinmann, Eike; Pietschmann, Thomas
Abstract:
Due to the obligatory intracellular lifestyle of viruses, cell culture systems for efficient viral propagation are crucial to obtain a detailed understanding of the virus-host cell interaction. For hepatitis C virus (HCV) the development of permissive and authentic culture models continues to be a challenging task. The first efforts to culture HCV had limited success and range back to before the virus was molecularly cloned in 1989. Since then several major breakthroughs have gradually overcome limitations in culturing the virus and sequentially permitted analysis of viral RNA replication, cell entry, and ultimately the complete replication cycle in cultured cells in 2005. Until today, basic and applied HCV research greatly benefit from these tremendous efforts which spurred multiple complementary cell-based model systems for distinct steps of the HCV replication cycle. When used in combination they now permit deep insights into the fascinating biology of HCV and its interplay with the host cell. In fact, drug development has been much facilitated and our understanding of the molecular determinants of HCV replication has grown in parallel to these advances. Building on this groundwork and further refining our cellular models to better mimic the architecture, polarization and differentiation of natural hepatocytes should reveal novel unique aspects of HCV replication. Ultimately, models to culture primary HCV isolates across all genotypes may teach us important new lessons about viral functional adaptations that have evolved in exchange with its human host and that may explain the variable natural course of hepatitis C.
Affiliation:
Helmholtz Centre for Infection Research, Hannover, Germany.
Citation:
Cell culture systems for hepatitis C virus. 2013, 369:17-48 Curr. Top. Microbiol. Immunol.
Journal:
Current topics in microbiology and immunology
Issue Date:
2013
URI:
http://hdl.handle.net/10033/295974
DOI:
10.1007/978-3-642-27340-7_2
PubMed ID:
23463196
Type:
Book chapter
Language:
en
ISSN:
0070-217X
Appears in Collections:
publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorSteinmann, Eikeen_GB
dc.contributor.authorPietschmann, Thomasen_GB
dc.date.accessioned2013-07-15T11:00:42Z-
dc.date.available2013-07-15T11:00:42Z-
dc.date.issued2013-
dc.identifier.citationCell culture systems for hepatitis C virus. 2013, 369:17-48 Curr. Top. Microbiol. Immunol.en_GB
dc.identifier.issn0070-217X-
dc.identifier.pmid23463196-
dc.identifier.doi10.1007/978-3-642-27340-7_2-
dc.identifier.urihttp://hdl.handle.net/10033/295974-
dc.description.abstractDue to the obligatory intracellular lifestyle of viruses, cell culture systems for efficient viral propagation are crucial to obtain a detailed understanding of the virus-host cell interaction. For hepatitis C virus (HCV) the development of permissive and authentic culture models continues to be a challenging task. The first efforts to culture HCV had limited success and range back to before the virus was molecularly cloned in 1989. Since then several major breakthroughs have gradually overcome limitations in culturing the virus and sequentially permitted analysis of viral RNA replication, cell entry, and ultimately the complete replication cycle in cultured cells in 2005. Until today, basic and applied HCV research greatly benefit from these tremendous efforts which spurred multiple complementary cell-based model systems for distinct steps of the HCV replication cycle. When used in combination they now permit deep insights into the fascinating biology of HCV and its interplay with the host cell. In fact, drug development has been much facilitated and our understanding of the molecular determinants of HCV replication has grown in parallel to these advances. Building on this groundwork and further refining our cellular models to better mimic the architecture, polarization and differentiation of natural hepatocytes should reveal novel unique aspects of HCV replication. Ultimately, models to culture primary HCV isolates across all genotypes may teach us important new lessons about viral functional adaptations that have evolved in exchange with its human host and that may explain the variable natural course of hepatitis C.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Current topics in microbiology and immunologyen_GB
dc.titleCell culture systems for hepatitis C virus.en
dc.typeBook chapteren
dc.contributor.departmentHelmholtz Centre for Infection Research, Hannover, Germany.en_GB
dc.identifier.journalCurrent topics in microbiology and immunologyen_GB

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