Description of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov.

2.50
Hdl Handle:
http://hdl.handle.net/10033/296284
Title:
Description of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov.
Authors:
Jogler, Mareike; Chen, Hong; Simon, Julia; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Busse, Hans-Jürgen; Klenk, Hans-Peter; Tindall, Brian J; Overmann, Jörg
Abstract:
A previously undescribed aerobic, non-sporulating bacterium, strain G1A_585(T), was isolated from an oligotrophic freshwater lake in Bavaria, Germany. The rod-shaped cells were Gram-stain-negative and non-motile. Based on 16S rRNA gene sequence similarity, strain G1A_585(T) was a member of the family Sphingomonadaceae and shared <95.2 % similarity with type strains of all members of the most closely related genus, Sphingopyxis. Phyogenetically, the isolate shared a root with strains of three marine species, Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T). The polar lipids of strain G1A_585(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, sphingoglycolipids, three glycolipids and one unknown lipid. Ubiquinone-10 was the dominant quinone (93.1 %) and ubiquinone-9 (6.5 %) was also detected. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 38.2 %); C16 : 1ω7c (33.6 %) and C14 : 0 2-OH (17.8 %). The major polyamine was spermidine and traces of 1,3-diaminopropane, putrescine and spermine were also detected. The DNA G+C content of strain G1A_585(T) was 55.7 mol% and the isolate was oxidase- and catalase-positive. Based on the phylogenetic relationship, the low DNA G+C content compared with most other members of the genus Sphingopyxis and the presence of signature nucleotides in the 16S rRNA gene sequence, a novel species in a new genus and species, Sphingorhabdus planktonica gen. nov., sp. nov., is proposed; the type strain of Sphingorhabdus planktonica is G1A_585(T) ( = DSM 25081(T)  = LMG 26646(T)). Because Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T) form a phylogenetic group together with strain G1A_585(T) that is clearly separated from all other known Sphingopyxis strains and share signature nucleotides, these three Sphingopyxis strains are reclassified as members of the proposed novel genus Sphingorhabdus: Sphingorhabdus flavimaris comb. nov. (type strain SW-151(T) = DSM 16223(T) = KCTC 12232(T)), Sphingorhabdus marina comb. nov. (type strain FR1087(T) = DSM 22363(T) = IMSNU 14132(T) = KCTC 12763(T) = JCM 14161(T)) and Sphingorhabdus litoris comb. nov. (type strain FR1093(T) = DSM 22379(T) = IMSNU 14133(T) = KCTC 12764(T) = JCM 14162(T)).
Affiliation:
Bereich Mikrobiologie, Biologie I, Ludwig-Maximilians-Universität München, Planegg, Martinsried, Germany.
Citation:
Description of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov. 2013, 63 (Pt 4):1342-9 Int. J. Syst. Evol. Microbiol.
Journal:
International journal of systematic and evolutionary microbiology
Issue Date:
Apr-2013
URI:
http://hdl.handle.net/10033/296284
DOI:
10.1099/ijs.0.043133-0
PubMed ID:
22798658
Type:
Article
Language:
en
ISSN:
1466-5034
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorJogler, Mareikeen_GB
dc.contributor.authorChen, Hongen_GB
dc.contributor.authorSimon, Juliaen_GB
dc.contributor.authorRohde, Manfreden_GB
dc.contributor.authorBusse, Hans-Jürgenen_GB
dc.contributor.authorKlenk, Hans-Peteren_GB
dc.contributor.authorTindall, Brian Jen_GB
dc.contributor.authorOvermann, Jörgen_GB
dc.date.accessioned2013-07-17T10:50:20Zen
dc.date.available2013-07-17T10:50:20Zen
dc.date.issued2013-04en
dc.identifier.citationDescription of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov. 2013, 63 (Pt 4):1342-9 Int. J. Syst. Evol. Microbiol.en_GB
dc.identifier.issn1466-5034en
dc.identifier.pmid22798658en
dc.identifier.doi10.1099/ijs.0.043133-0en
dc.identifier.urihttp://hdl.handle.net/10033/296284en
dc.description.abstractA previously undescribed aerobic, non-sporulating bacterium, strain G1A_585(T), was isolated from an oligotrophic freshwater lake in Bavaria, Germany. The rod-shaped cells were Gram-stain-negative and non-motile. Based on 16S rRNA gene sequence similarity, strain G1A_585(T) was a member of the family Sphingomonadaceae and shared <95.2 % similarity with type strains of all members of the most closely related genus, Sphingopyxis. Phyogenetically, the isolate shared a root with strains of three marine species, Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T). The polar lipids of strain G1A_585(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, sphingoglycolipids, three glycolipids and one unknown lipid. Ubiquinone-10 was the dominant quinone (93.1 %) and ubiquinone-9 (6.5 %) was also detected. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 38.2 %); C16 : 1ω7c (33.6 %) and C14 : 0 2-OH (17.8 %). The major polyamine was spermidine and traces of 1,3-diaminopropane, putrescine and spermine were also detected. The DNA G+C content of strain G1A_585(T) was 55.7 mol% and the isolate was oxidase- and catalase-positive. Based on the phylogenetic relationship, the low DNA G+C content compared with most other members of the genus Sphingopyxis and the presence of signature nucleotides in the 16S rRNA gene sequence, a novel species in a new genus and species, Sphingorhabdus planktonica gen. nov., sp. nov., is proposed; the type strain of Sphingorhabdus planktonica is G1A_585(T) ( = DSM 25081(T)  = LMG 26646(T)). Because Sphingopyxis flavimaris DSM 16223(T), Sphingopyxis marina DSM 22363(T) and Sphingopyxis litoris DSM 22379(T) form a phylogenetic group together with strain G1A_585(T) that is clearly separated from all other known Sphingopyxis strains and share signature nucleotides, these three Sphingopyxis strains are reclassified as members of the proposed novel genus Sphingorhabdus: Sphingorhabdus flavimaris comb. nov. (type strain SW-151(T) = DSM 16223(T) = KCTC 12232(T)), Sphingorhabdus marina comb. nov. (type strain FR1087(T) = DSM 22363(T) = IMSNU 14132(T) = KCTC 12763(T) = JCM 14161(T)) and Sphingorhabdus litoris comb. nov. (type strain FR1093(T) = DSM 22379(T) = IMSNU 14133(T) = KCTC 12764(T) = JCM 14162(T)).en_GB
dc.language.isoenen
dc.rightsArchived with thanks to International journal of systematic and evolutionary microbiologyen_GB
dc.subject.meshBacterial Typing Techniquesen_GB
dc.subject.meshBase Compositionen_GB
dc.subject.meshDNA, Bacterialen_GB
dc.subject.meshFatty Acidsen_GB
dc.subject.meshFresh Wateren_GB
dc.subject.meshGermanyen_GB
dc.subject.meshLakesen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshPhylogenyen_GB
dc.subject.meshRNA, Ribosomal, 16Sen_GB
dc.subject.meshSequence Analysis, DNAen_GB
dc.subject.meshSpermidineen_GB
dc.subject.meshSphingomonadaceaeen_GB
dc.subject.meshUbiquinoneen_GB
dc.subject.meshWater Microbiologyen_GB
dc.titleDescription of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov.en
dc.typeArticleen
dc.contributor.departmentBereich Mikrobiologie, Biologie I, Ludwig-Maximilians-Universität München, Planegg, Martinsried, Germany.en_GB
dc.identifier.journalInternational journal of systematic and evolutionary microbiologyen_GB
This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.