Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae.

2.50
Hdl Handle:
http://hdl.handle.net/10033/297441
Title:
Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae.
Authors:
Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Lünsdorf, Heinrich; Vancanneyt, Marc; Smit, John
Abstract:
Cauliform bacteria are prosthecate bacteria often specialized for oligotrophic environments. A polyphasic approach, comprising 16S rRNA gene sequencing, lipid analysis and salt tolerance characterizations, was used to clarify the taxonomy of one isolate, strain MCS 33(T), obtained from above the hot water plume of a deep-sea hydrothermal vent near Vancouver island, Canada. Cells contained no detectable phospholipids or sulpholipids, but did contain 1,2-di-O-acyl-3-O-α-D-glucopyranosylglycerol, 1,2-di-O-acyl-3-O-α-D-glucopyranuronosylglycerol and the novel lipid 1,2-di-O-acyl-3-[O-α-D-glucopyranuronosyl]glycerol-6'-N-glycine. It is assumed that the various glucoronosyl lipids are replacing, at least partially, the phospholipids in their various tasks in the cell cycle. The G+C content of the genomic DNA of strain MCS 33(T) was 62.8 mol%, and Q10 was the predominant respiratory ubiquinone. The 16S rRNA gene sequence of this chemoheterotrophic, aerobic, moderately halophilic strain showed only a low similarity of 94.4% to that of Oceanicaulis alexandrii C116-18(T), and both strains also differed based on their lipids. Although the novel strain was isolated from seawater sampled near a hydrothermal vent, its optimum temperature for growth was 30 °C. The main cellular fatty acids were C18:1ω7c, C18:0 and the unknown fatty acid ECL 11.798, and the main hydroxy fatty acid was C12:0 3-OH. The strain is proposed to represent a novel species of a new genus, Glycocaulis abyssi gen. nov., sp. nov. The type strain of the type species is MCS 33(T) (=LMG 27140(T)=CCUG 62981(T)).
Affiliation:
Helmholtz Center for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany. wolf-rainer.abraham@helmholtz-hzi.de
Citation:
Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae. 2013, 63 (Pt 6):2207-15 Int. J. Syst. Evol. Microbiol.
Journal:
International journal of systematic and evolutionary microbiology
Issue Date:
Jun-2013
URI:
http://hdl.handle.net/10033/297441
DOI:
10.1099/ijs.0.047894-0
PubMed ID:
23148094
Type:
Article
Language:
en
ISSN:
1466-5034
Appears in Collections:
publications of the research group chemical microbiology (CMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorAbraham, Wolf-Raineren_GB
dc.contributor.authorLünsdorf, Heinrichen_GB
dc.contributor.authorVancanneyt, Marcen_GB
dc.contributor.authorSmit, Johnen_GB
dc.date.accessioned2013-08-06T08:46:22Z-
dc.date.available2013-08-06T08:46:22Z-
dc.date.issued2013-06-
dc.identifier.citationCauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae. 2013, 63 (Pt 6):2207-15 Int. J. Syst. Evol. Microbiol.en_GB
dc.identifier.issn1466-5034-
dc.identifier.pmid23148094-
dc.identifier.doi10.1099/ijs.0.047894-0-
dc.identifier.urihttp://hdl.handle.net/10033/297441-
dc.description.abstractCauliform bacteria are prosthecate bacteria often specialized for oligotrophic environments. A polyphasic approach, comprising 16S rRNA gene sequencing, lipid analysis and salt tolerance characterizations, was used to clarify the taxonomy of one isolate, strain MCS 33(T), obtained from above the hot water plume of a deep-sea hydrothermal vent near Vancouver island, Canada. Cells contained no detectable phospholipids or sulpholipids, but did contain 1,2-di-O-acyl-3-O-α-D-glucopyranosylglycerol, 1,2-di-O-acyl-3-O-α-D-glucopyranuronosylglycerol and the novel lipid 1,2-di-O-acyl-3-[O-α-D-glucopyranuronosyl]glycerol-6'-N-glycine. It is assumed that the various glucoronosyl lipids are replacing, at least partially, the phospholipids in their various tasks in the cell cycle. The G+C content of the genomic DNA of strain MCS 33(T) was 62.8 mol%, and Q10 was the predominant respiratory ubiquinone. The 16S rRNA gene sequence of this chemoheterotrophic, aerobic, moderately halophilic strain showed only a low similarity of 94.4% to that of Oceanicaulis alexandrii C116-18(T), and both strains also differed based on their lipids. Although the novel strain was isolated from seawater sampled near a hydrothermal vent, its optimum temperature for growth was 30 °C. The main cellular fatty acids were C18:1ω7c, C18:0 and the unknown fatty acid ECL 11.798, and the main hydroxy fatty acid was C12:0 3-OH. The strain is proposed to represent a novel species of a new genus, Glycocaulis abyssi gen. nov., sp. nov. The type strain of the type species is MCS 33(T) (=LMG 27140(T)=CCUG 62981(T)).en_GB
dc.language.isoenen
dc.rightsArchived with thanks to International journal of systematic and evolutionary microbiologyen_GB
dc.titleCauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Center for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany. wolf-rainer.abraham@helmholtz-hzi.deen_GB
dc.identifier.journalInternational journal of systematic and evolutionary microbiologyen_GB

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