2.50
Hdl Handle:
http://hdl.handle.net/10033/298067
Title:
Thermostability of seven hepatitis C virus genotypes in vitro and in vivo.
Authors:
Doerrbecker, J; Meuleman, P; Kang, J; Riebesehl, N; Wilhelm, C; Friesland, M; Pfaender, S; Steinmann, J; Pietschmann, T; Steinmann, E
Abstract:
Hepatitis C virus (HCV) is transmitted primarily through percutaneous exposure to contaminated blood especially in healthcare settings and among people who inject drugs. The environmental stability of HCV has been extrapolated from studies with the bovine viral diarrhoea virus or was so far only addressed with HCV genotype 2a viruses. The aim of this study was to compare the environmental and thermostability of all so far known seven HCV genotypes in vitro and in vivo. Incubation experiments at room temperature revealed that all HCV genotypes showed similar environmental stabilities in suspension with viral infectivity detectable for up to 28 days. The risk of HCV infection may not accurately be reflected by determination of HCV RNA levels. However, viral stability and transmission risks assessed from in vitro experiments correlated with viral infectivity in transgenic mice containing human liver xenografts. A reduced viral stability for up to 2 days was observed at 37 °C with comparable decays for all HCV genotypes confirmed by thermodynamic analysis. These results demonstrate that different HCV genotypes possess comparable stability in the environment and that noninfectious particles after incubation in vitro do not cause infection in an HCV in vivo model. These findings are important for estimation of HCV cross-transmission in the environment and indicate that different HCV genotypes do not display an altered stability or resistance at certain temperatures.
Affiliation:
Division of Experimental Virology, Twincore Center for Experimental and Clinical Infection Research, Feodor-Lynen-Straße 7-9, 30625 Hannover, Germany.
Citation:
Thermostability of seven hepatitis C virus genotypes in vitro and in vivo. 2013, 20 (7):478-85 J. Viral Hepat.
Journal:
Journal of viral hepatitis
Issue Date:
Jul-2013
URI:
http://hdl.handle.net/10033/298067
DOI:
10.1111/jvh.12055
PubMed ID:
23730841
Type:
Article
Language:
en
ISSN:
1365-2893
Appears in Collections:
publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorDoerrbecker, Jen_GB
dc.contributor.authorMeuleman, Pen_GB
dc.contributor.authorKang, Jen_GB
dc.contributor.authorRiebesehl, Nen_GB
dc.contributor.authorWilhelm, Cen_GB
dc.contributor.authorFriesland, Men_GB
dc.contributor.authorPfaender, Sen_GB
dc.contributor.authorSteinmann, Jen_GB
dc.contributor.authorPietschmann, Ten_GB
dc.contributor.authorSteinmann, Een_GB
dc.date.accessioned2013-08-13T09:48:31Zen
dc.date.available2013-08-13T09:48:31Zen
dc.date.issued2013-07en
dc.identifier.citationThermostability of seven hepatitis C virus genotypes in vitro and in vivo. 2013, 20 (7):478-85 J. Viral Hepat.en_GB
dc.identifier.issn1365-2893en
dc.identifier.pmid23730841en
dc.identifier.doi10.1111/jvh.12055en
dc.identifier.urihttp://hdl.handle.net/10033/298067en
dc.description.abstractHepatitis C virus (HCV) is transmitted primarily through percutaneous exposure to contaminated blood especially in healthcare settings and among people who inject drugs. The environmental stability of HCV has been extrapolated from studies with the bovine viral diarrhoea virus or was so far only addressed with HCV genotype 2a viruses. The aim of this study was to compare the environmental and thermostability of all so far known seven HCV genotypes in vitro and in vivo. Incubation experiments at room temperature revealed that all HCV genotypes showed similar environmental stabilities in suspension with viral infectivity detectable for up to 28 days. The risk of HCV infection may not accurately be reflected by determination of HCV RNA levels. However, viral stability and transmission risks assessed from in vitro experiments correlated with viral infectivity in transgenic mice containing human liver xenografts. A reduced viral stability for up to 2 days was observed at 37 °C with comparable decays for all HCV genotypes confirmed by thermodynamic analysis. These results demonstrate that different HCV genotypes possess comparable stability in the environment and that noninfectious particles after incubation in vitro do not cause infection in an HCV in vivo model. These findings are important for estimation of HCV cross-transmission in the environment and indicate that different HCV genotypes do not display an altered stability or resistance at certain temperatures.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of viral hepatitisen_GB
dc.titleThermostability of seven hepatitis C virus genotypes in vitro and in vivo.en
dc.typeArticleen
dc.contributor.departmentDivision of Experimental Virology, Twincore Center for Experimental and Clinical Infection Research, Feodor-Lynen-Straße 7-9, 30625 Hannover, Germany.en_GB
dc.identifier.journalJournal of viral hepatitisen_GB

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