2.50
Hdl Handle:
http://hdl.handle.net/10033/299523
Title:
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
Authors:
Kube, Michael; Chernikova, Tatyana N; Al-Ramahi, Yamal; Beloqui, Ana; Lopez-Cortez, Nieves; Guazzaroni, María-Eugenia; Heipieper, Hermann J; Klages, Sven; Kotsyurbenko, Oleg R; Langer, Ines; Nechitaylo, Taras Y; Lünsdorf, Heinrich; Fernández, Marisol; Juárez, Silvia; Ciordia, Sergio; Singer, Alexander; Kagan, Olga; Egorova, Olga; Alain Petit, Pierre; Stogios, Peter; Kim, Youngchang; Tchigvintsev, Anatoli; Flick, Robert; Denaro, Renata; Genovese, Maria; Albar, Juan P; Reva, Oleg N; Martínez-Gomariz, Montserrat; Tran, Hai; Ferrer, Manuel; Savchenko, Alexei; Yakunin, Alexander F; Yakimov, Michail M; Golyshina, Olga V; Reinhardt, Richard; Golyshin, Peter N
Abstract:
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis-the paradigm of mesophilic hydrocarbonoclastic bacteria-O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.
Affiliation:
1] Max-Planck Institute for Molecular Genetics, Berlin-Dahlem D-14195, Germany [2] Section Phytomedicine, Department of Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin-Dahlem D-14195, Germany.
Citation:
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. 2013, 4:2156 Nat Commun
Journal:
Nature communications
Issue Date:
23-Jul-2013
URI:
http://hdl.handle.net/10033/299523
DOI:
10.1038/ncomms3156
PubMed ID:
23877221
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValue Language
dc.contributor.authorKube, Michaelen_GB
dc.contributor.authorChernikova, Tatyana Nen_GB
dc.contributor.authorAl-Ramahi, Yamalen_GB
dc.contributor.authorBeloqui, Anaen_GB
dc.contributor.authorLopez-Cortez, Nievesen_GB
dc.contributor.authorGuazzaroni, María-Eugeniaen_GB
dc.contributor.authorHeipieper, Hermann Jen_GB
dc.contributor.authorKlages, Svenen_GB
dc.contributor.authorKotsyurbenko, Oleg Ren_GB
dc.contributor.authorLanger, Inesen_GB
dc.contributor.authorNechitaylo, Taras Yen_GB
dc.contributor.authorLünsdorf, Heinrichen_GB
dc.contributor.authorFernández, Marisolen_GB
dc.contributor.authorJuárez, Silviaen_GB
dc.contributor.authorCiordia, Sergioen_GB
dc.contributor.authorSinger, Alexanderen_GB
dc.contributor.authorKagan, Olgaen_GB
dc.contributor.authorEgorova, Olgaen_GB
dc.contributor.authorAlain Petit, Pierreen_GB
dc.contributor.authorStogios, Peteren_GB
dc.contributor.authorKim, Youngchangen_GB
dc.contributor.authorTchigvintsev, Anatolien_GB
dc.contributor.authorFlick, Roberten_GB
dc.contributor.authorDenaro, Renataen_GB
dc.contributor.authorGenovese, Mariaen_GB
dc.contributor.authorAlbar, Juan Pen_GB
dc.contributor.authorReva, Oleg Nen_GB
dc.contributor.authorMartínez-Gomariz, Montserraten_GB
dc.contributor.authorTran, Haien_GB
dc.contributor.authorFerrer, Manuelen_GB
dc.contributor.authorSavchenko, Alexeien_GB
dc.contributor.authorYakunin, Alexander Fen_GB
dc.contributor.authorYakimov, Michail Men_GB
dc.contributor.authorGolyshina, Olga Ven_GB
dc.contributor.authorReinhardt, Richarden_GB
dc.contributor.authorGolyshin, Peter Nen_GB
dc.date.accessioned2013-08-22T14:07:55Z-
dc.date.available2013-08-22T14:07:55Z-
dc.date.issued2013-07-23-
dc.identifier.citationGenome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. 2013, 4:2156 Nat Communen_GB
dc.identifier.issn2041-1723-
dc.identifier.pmid23877221-
dc.identifier.doi10.1038/ncomms3156-
dc.identifier.urihttp://hdl.handle.net/10033/299523-
dc.description.abstractUbiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis-the paradigm of mesophilic hydrocarbonoclastic bacteria-O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Nature communicationsen_GB
dc.titleGenome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.en
dc.typeArticleen
dc.contributor.department1] Max-Planck Institute for Molecular Genetics, Berlin-Dahlem D-14195, Germany [2] Section Phytomedicine, Department of Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin-Dahlem D-14195, Germany.en_GB
dc.identifier.journalNature communicationsen_GB

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