2.50
Hdl Handle:
http://hdl.handle.net/10033/301998
Title:
Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.
Authors:
Schwerk, Johannes; Köster, Mario; Hauser, Hansjörg; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Fulde, Marcus; Hornef, Mathias W; May, Tobias
Abstract:
Cell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.
Affiliation:
Department of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions. 2013, 8 (8):e72700 PLoS ONE
Journal:
PloS one
Issue Date:
2013
URI:
http://hdl.handle.net/10033/301998
DOI:
10.1371/journal.pone.0072700
PubMed ID:
23940817
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchwerk, Johannesen_GB
dc.contributor.authorKöster, Marioen_GB
dc.contributor.authorHauser, Hansjörgen_GB
dc.contributor.authorRohde, Manfreden_GB
dc.contributor.authorFulde, Marcusen_GB
dc.contributor.authorHornef, Mathias Wen_GB
dc.contributor.authorMay, Tobiasen_GB
dc.date.accessioned2013-09-20T12:05:26Zen
dc.date.available2013-09-20T12:05:26Zen
dc.date.issued2013en
dc.identifier.citationGeneration of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions. 2013, 8 (8):e72700 PLoS ONEen_GB
dc.identifier.issn1932-6203en
dc.identifier.pmid23940817en
dc.identifier.doi10.1371/journal.pone.0072700en
dc.identifier.urihttp://hdl.handle.net/10033/301998en
dc.description.abstractCell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleGeneration of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.en
dc.typeArticleen
dc.contributor.departmentDepartment of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany.en_GB
dc.identifier.journalPloS oneen_GB

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