2.50
Hdl Handle:
http://hdl.handle.net/10033/302501
Title:
Cytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein.
Authors:
Wolters, Manuel; Boyle, Erin C; Lardong, Kerstin; Trülzsch, Konrad; Steffen, Anika; Rottner, Klemens; Ruckdeschel, Klaus; Aepfelbacher, Martin
Abstract:
Pathogenic Yersinia spp. translocate the effectors YopT, YopE, and YopO/YpkA into target cells to inactivate Rho family GTP-binding proteins and block immune responses. Some Yersinia spp. also secrete the Rho protein activator cytotoxic necrotizing factor-Y (CNF-Y), but it has been unclear how the bacteria may benefit from Rho protein activation. We show here that CNF-Y increases Yop translocation in Yersinia enterocolitica-infected cells up to 5-fold. CNF-Y strongly activated RhoA and also delayed in time Rac1 and Cdc42, but when individually expressed, constitutively active mutants of Rac1, but not of RhoA, increased Yop translocation. Consistently, knock-out or knockdown of Rac1 but not of RhoA, -B, or -C inhibited Yersinia effector translocation in CNF-Y-treated and control cells. Activation or knockdown of Cdc42 also affected Yop translocation but much less efficiently than Rac. The increase in Yop translocation induced by CNF-Y was essentially independent of the presence of YopE, YopT, or YopO in the infecting Yersinia strain, indicating that none of the Yops reported to inhibit translocation could reverse the CNF-Y effect. In summary, the CNF-Y activity of Yersinia strongly enhances Yop translocation through activation of Rac.
Affiliation:
From the Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Citation:
Cytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein. 2013, 288 (32):23543-53 J. Biol. Chem.
Journal:
The Journal of biological chemistry
Issue Date:
9-Aug-2013
URI:
http://hdl.handle.net/10033/302501
DOI:
10.1074/jbc.M112.448662
PubMed ID:
23803609
Type:
Article
Language:
en
ISSN:
1083-351X
Appears in Collections:
Publications of RG Cytoskeleton Dynamics (CYD)

Full metadata record

DC FieldValue Language
dc.contributor.authorWolters, Manuelen
dc.contributor.authorBoyle, Erin Cen
dc.contributor.authorLardong, Kerstinen
dc.contributor.authorTrülzsch, Konraden
dc.contributor.authorSteffen, Anikaen
dc.contributor.authorRottner, Klemensen
dc.contributor.authorRuckdeschel, Klausen
dc.contributor.authorAepfelbacher, Martinen
dc.date.accessioned2013-09-30T08:44:15Z-
dc.date.available2013-09-30T08:44:15Z-
dc.date.issued2013-08-09-
dc.identifier.citationCytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein. 2013, 288 (32):23543-53 J. Biol. Chem.en
dc.identifier.issn1083-351X-
dc.identifier.pmid23803609-
dc.identifier.doi10.1074/jbc.M112.448662-
dc.identifier.urihttp://hdl.handle.net/10033/302501-
dc.description.abstractPathogenic Yersinia spp. translocate the effectors YopT, YopE, and YopO/YpkA into target cells to inactivate Rho family GTP-binding proteins and block immune responses. Some Yersinia spp. also secrete the Rho protein activator cytotoxic necrotizing factor-Y (CNF-Y), but it has been unclear how the bacteria may benefit from Rho protein activation. We show here that CNF-Y increases Yop translocation in Yersinia enterocolitica-infected cells up to 5-fold. CNF-Y strongly activated RhoA and also delayed in time Rac1 and Cdc42, but when individually expressed, constitutively active mutants of Rac1, but not of RhoA, increased Yop translocation. Consistently, knock-out or knockdown of Rac1 but not of RhoA, -B, or -C inhibited Yersinia effector translocation in CNF-Y-treated and control cells. Activation or knockdown of Cdc42 also affected Yop translocation but much less efficiently than Rac. The increase in Yop translocation induced by CNF-Y was essentially independent of the presence of YopE, YopT, or YopO in the infecting Yersinia strain, indicating that none of the Yops reported to inhibit translocation could reverse the CNF-Y effect. In summary, the CNF-Y activity of Yersinia strongly enhances Yop translocation through activation of Rac.en
dc.language.isoenen
dc.rightsArchived with thanks to The Journal of biological chemistryen
dc.titleCytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein.en
dc.typeArticleen
dc.contributor.departmentFrom the Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.en
dc.identifier.journalThe Journal of biological chemistryen

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