Electron microscopic, genetic and protein expression analyses of Helicobacter acinonychis strains from a Bengal tiger.

2.50
Hdl Handle:
http://hdl.handle.net/10033/304811
Title:
Electron microscopic, genetic and protein expression analyses of Helicobacter acinonychis strains from a Bengal tiger.
Authors:
Tegtmeyer, Nicole; Rivas Traverso, Francisco; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Oyarzabal, Omar A; Lehn, Norbert; Schneider-Brachert, Wulf; Ferrero, Richard L; Fox, James G; Berg, Douglas E; Backert, Steffen
Abstract:
Colonization by Helicobacter species is commonly noted in many mammals. These infections often remain unrecognized, but can cause severe health complications or more subtle host immune perturbations. The aim of this study was to isolate and characterize putative novel Helicobacter spp. from Bengal tigers in Thailand. Morphological investigation (Gram-staining and electron microscopy) and genetic studies (16SrRNA, 23SrRNA, flagellin, urease and prophage gene analyses, RAPD DNA fingerprinting and restriction fragment polymorphisms) as well as Western blotting were used to characterize the isolated Helicobacters. Electron microscopy revealed spiral-shaped bacteria, which varied in length (2.5-6 µm) and contained up to four monopolar sheathed flagella. The 16SrRNA, 23SrRNA, sequencing and protein expression analyses identified novel H. acinonychis isolates closely related to H. pylori. These Asian isolates are genetically very similar to H. acinonychis strains of other big cats (cheetahs, lions, lion-tiger hybrid and other tigers) from North America and Europe, which is remarkable in the context of the great genetic diversity among worldwide H. pylori strains. We also found by immunoblotting that the Bengal tiger isolates express UreaseA/B, flagellin, BabA adhesin, neutrophil-activating protein NapA, HtrA protease, γ-glutamyl-transpeptidase GGT, Slt lytic transglycosylase and two DNA transfer relaxase orthologs that were known from H. pylori, but not the cag pathogenicity island, nor CagA, VacA, SabA, DupA or OipA proteins. These results give fresh insights into H. acinonychis genetics and the expression of potential pathogenicity-associated factors and their possible pathophysiological relevance in related gastric infections.
Affiliation:
Institute of Medical Microbiology, Otto von Guericke University Magdeburg, Magdeburg, Germany.
Citation:
Electron microscopic, genetic and protein expression analyses of Helicobacter acinonychis strains from a Bengal tiger. 2013, 8 (8):e71220 PLoS ONE
Journal:
PloS one
Issue Date:
2013
URI:
http://hdl.handle.net/10033/304811
DOI:
10.1371/journal.pone.0071220
PubMed ID:
23940723
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorTegtmeyer, Nicoleen
dc.contributor.authorRivas Traverso, Franciscoen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorOyarzabal, Omar Aen
dc.contributor.authorLehn, Norberten
dc.contributor.authorSchneider-Brachert, Wulfen
dc.contributor.authorFerrero, Richard Len
dc.contributor.authorFox, James Gen
dc.contributor.authorBerg, Douglas Een
dc.contributor.authorBackert, Steffenen
dc.date.accessioned2013-10-31T10:23:00Zen
dc.date.available2013-10-31T10:23:00Zen
dc.date.issued2013en
dc.identifier.citationElectron microscopic, genetic and protein expression analyses of Helicobacter acinonychis strains from a Bengal tiger. 2013, 8 (8):e71220 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid23940723en
dc.identifier.doi10.1371/journal.pone.0071220en
dc.identifier.urihttp://hdl.handle.net/10033/304811en
dc.description.abstractColonization by Helicobacter species is commonly noted in many mammals. These infections often remain unrecognized, but can cause severe health complications or more subtle host immune perturbations. The aim of this study was to isolate and characterize putative novel Helicobacter spp. from Bengal tigers in Thailand. Morphological investigation (Gram-staining and electron microscopy) and genetic studies (16SrRNA, 23SrRNA, flagellin, urease and prophage gene analyses, RAPD DNA fingerprinting and restriction fragment polymorphisms) as well as Western blotting were used to characterize the isolated Helicobacters. Electron microscopy revealed spiral-shaped bacteria, which varied in length (2.5-6 µm) and contained up to four monopolar sheathed flagella. The 16SrRNA, 23SrRNA, sequencing and protein expression analyses identified novel H. acinonychis isolates closely related to H. pylori. These Asian isolates are genetically very similar to H. acinonychis strains of other big cats (cheetahs, lions, lion-tiger hybrid and other tigers) from North America and Europe, which is remarkable in the context of the great genetic diversity among worldwide H. pylori strains. We also found by immunoblotting that the Bengal tiger isolates express UreaseA/B, flagellin, BabA adhesin, neutrophil-activating protein NapA, HtrA protease, γ-glutamyl-transpeptidase GGT, Slt lytic transglycosylase and two DNA transfer relaxase orthologs that were known from H. pylori, but not the cag pathogenicity island, nor CagA, VacA, SabA, DupA or OipA proteins. These results give fresh insights into H. acinonychis genetics and the expression of potential pathogenicity-associated factors and their possible pathophysiological relevance in related gastric infections.en
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen
dc.titleElectron microscopic, genetic and protein expression analyses of Helicobacter acinonychis strains from a Bengal tiger.en
dc.typeArticleen
dc.contributor.departmentInstitute of Medical Microbiology, Otto von Guericke University Magdeburg, Magdeburg, Germany.en
dc.identifier.journalPloS oneen

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