DegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides.

2.50
Hdl Handle:
http://hdl.handle.net/10033/306441
Title:
DegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides.
Authors:
Nuss, Aaron M; Adnan, Fazal; Weber, Lennart; Berghoff, Bork A; Glaeser, Jens; Klug, Gabriele
Abstract:
Singlet oxygen ((1)O2) is the main agent of photooxidative stress and is generated by photosensitizers as (bacterio)chlorophylls. It leads to the damage of cellular macromolecules and therefore photosynthetic organisms have to mount an adaptive response to (1)O2 formation. A major player of the photooxidative stress response in Rhodobacter sphaeroides is the alternative sigma factor RpoE, which is inactivated under non-stress conditions by its cognate anti-sigma factor ChrR. By using random mutagenesis we identified RSP_1090 to be required for full activation of the RpoE response under (1)O2 stress, but not under organic peroxide stress. In this study we show that both RSP_1090 and RSP_1091 are required for full resistance towards (1)O2. Moreover, we revealed that the DegS and RseP homologs RSP_3242 and RSP_2710 contribute to (1)O2 resistance and promote ChrR proteolysis. The RpoE signaling pathway in R. sphaeroides is therefore highly similar to that of Escherichia coli, although very different anti-sigma factors control RpoE activity. Based on the acquired results, the current model for RpoE activation in response to (1)O2 exposure in R. sphaeroides was extended.
Affiliation:
Institute of Microbiology and Molecular Biology, Giessen University, Giessen, Germany ; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
DegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides. 2013, 8 (11):e79520 PLoS ONE
Journal:
PloS one
Issue Date:
2013
URI:
http://hdl.handle.net/10033/306441
DOI:
10.1371/journal.pone.0079520
PubMed ID:
24223961
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorAdnan, Fazalen
dc.contributor.authorWeber, Lennarten
dc.contributor.authorBerghoff, Bork Aen
dc.contributor.authorGlaeser, Jensen
dc.contributor.authorKlug, Gabrieleen
dc.date.accessioned2013-12-06T13:59:44Z-
dc.date.available2013-12-06T13:59:44Z-
dc.date.issued2013-
dc.identifier.citationDegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides. 2013, 8 (11):e79520 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid24223961-
dc.identifier.doi10.1371/journal.pone.0079520-
dc.identifier.urihttp://hdl.handle.net/10033/306441-
dc.description.abstractSinglet oxygen ((1)O2) is the main agent of photooxidative stress and is generated by photosensitizers as (bacterio)chlorophylls. It leads to the damage of cellular macromolecules and therefore photosynthetic organisms have to mount an adaptive response to (1)O2 formation. A major player of the photooxidative stress response in Rhodobacter sphaeroides is the alternative sigma factor RpoE, which is inactivated under non-stress conditions by its cognate anti-sigma factor ChrR. By using random mutagenesis we identified RSP_1090 to be required for full activation of the RpoE response under (1)O2 stress, but not under organic peroxide stress. In this study we show that both RSP_1090 and RSP_1091 are required for full resistance towards (1)O2. Moreover, we revealed that the DegS and RseP homologs RSP_3242 and RSP_2710 contribute to (1)O2 resistance and promote ChrR proteolysis. The RpoE signaling pathway in R. sphaeroides is therefore highly similar to that of Escherichia coli, although very different anti-sigma factors control RpoE activity. Based on the acquired results, the current model for RpoE activation in response to (1)O2 exposure in R. sphaeroides was extended.en
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen
dc.titleDegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides.en
dc.typeArticleen
dc.contributor.departmentInstitute of Microbiology and Molecular Biology, Giessen University, Giessen, Germany ; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalPloS oneen
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