Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays.

2.50
Hdl Handle:
http://hdl.handle.net/10033/306655
Title:
Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays.
Authors:
Lak, Aidin; Dieckhoff, Jan; Ludwig, Frank; Scholtyssek, Jan M; Goldmann, Oliver; Lünsdorf, Heinrich; Eberbeck, Dietmar; Kornowski, Andreas; Kraken, Mathias; Litterst, F J; Fiege, Kathrin; Mischnick, Petra; Schilling, Meinhard
Abstract:
Uniformly sized and shaped iron oxide nanoparticles with a mean size of 25 nm were synthesized via decomposition of iron-oleate. High resolution transmission electron microscopy and Mössbauer spectroscopy investigations revealed that the particles are spheres primarily composed of Fe3O4 with a small fraction of FeO. From Mössbauer and static magnetization measurements, it was deduced that the particles are superparamagnetic at room temperature. The hydrophobic particles were successfully transferred into water via PEGylation using nitrodopamine as an anchoring group. IR spectroscopy and thermogravimetric analysis showed the success and efficiency of the phase transfer reaction. After PEGylation, the particles retained monodispersity and their magnetic core remained intact as proven by photon cross-correlation spectroscopy, ac susceptibility, and transmission electron microscopy. The particle aqueous suspensions revealed excellent water stability over a month of monitoring and also against temperature up to 40 °C. The particles exhibited a moderate cytotoxic effect on in vitro cultured bone marrow-derived macrophages and no release of inflammatory or anti-inflammatory cytokines. The PEGylated particles were functionalized with Herceptin antibodies via a conjugation chemistry, their response to a rotating magnetic field was studied using a fluxgate-based setup and was compared with the one recorded for hydrophobic and PEGylated particles. The particle phase lag rose after labeling with Herceptin, indicating the successful conjugation of Herceptin antibodies to the particles.
Affiliation:
TU Braunschweig, Institute of Electrical Measurement and Fundamental Electrical Engineering, Hans-Sommer-St. 66, 38106 Braunschweig, Germany. a.lak@tu-bs.de f.ludwig@tu-bs.de.
Citation:
Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays. 2013, 5 (23):11447-55 Nanoscale
Journal:
Nanoscale
Issue Date:
7-Nov-2013
URI:
http://hdl.handle.net/10033/306655
DOI:
10.1039/c3nr02197a
PubMed ID:
24056778
Type:
Article
Language:
en
ISSN:
2040-3372
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorLak, Aidinen
dc.contributor.authorDieckhoff, Janen
dc.contributor.authorLudwig, Franken
dc.contributor.authorScholtyssek, Jan Men
dc.contributor.authorGoldmann, Oliveren
dc.contributor.authorLünsdorf, Heinrichen
dc.contributor.authorEberbeck, Dietmaren
dc.contributor.authorKornowski, Andreasen
dc.contributor.authorKraken, Mathiasen
dc.contributor.authorLitterst, F Jen
dc.contributor.authorFiege, Kathrinen
dc.contributor.authorMischnick, Petraen
dc.contributor.authorSchilling, Meinharden
dc.date.accessioned2013-12-10T10:41:16Z-
dc.date.available2013-12-10T10:41:16Z-
dc.date.issued2013-11-07-
dc.identifier.citationHighly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays. 2013, 5 (23):11447-55 Nanoscaleen
dc.identifier.issn2040-3372-
dc.identifier.pmid24056778-
dc.identifier.doi10.1039/c3nr02197a-
dc.identifier.urihttp://hdl.handle.net/10033/306655-
dc.description.abstractUniformly sized and shaped iron oxide nanoparticles with a mean size of 25 nm were synthesized via decomposition of iron-oleate. High resolution transmission electron microscopy and Mössbauer spectroscopy investigations revealed that the particles are spheres primarily composed of Fe3O4 with a small fraction of FeO. From Mössbauer and static magnetization measurements, it was deduced that the particles are superparamagnetic at room temperature. The hydrophobic particles were successfully transferred into water via PEGylation using nitrodopamine as an anchoring group. IR spectroscopy and thermogravimetric analysis showed the success and efficiency of the phase transfer reaction. After PEGylation, the particles retained monodispersity and their magnetic core remained intact as proven by photon cross-correlation spectroscopy, ac susceptibility, and transmission electron microscopy. The particle aqueous suspensions revealed excellent water stability over a month of monitoring and also against temperature up to 40 °C. The particles exhibited a moderate cytotoxic effect on in vitro cultured bone marrow-derived macrophages and no release of inflammatory or anti-inflammatory cytokines. The PEGylated particles were functionalized with Herceptin antibodies via a conjugation chemistry, their response to a rotating magnetic field was studied using a fluxgate-based setup and was compared with the one recorded for hydrophobic and PEGylated particles. The particle phase lag rose after labeling with Herceptin, indicating the successful conjugation of Herceptin antibodies to the particles.en
dc.language.isoenen
dc.rightsArchived with thanks to Nanoscaleen
dc.titleHighly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays.en
dc.typeArticleen
dc.contributor.departmentTU Braunschweig, Institute of Electrical Measurement and Fundamental Electrical Engineering, Hans-Sommer-St. 66, 38106 Braunschweig, Germany. a.lak@tu-bs.de f.ludwig@tu-bs.de.en
dc.identifier.journalNanoscaleen

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