2.50
Hdl Handle:
http://hdl.handle.net/10033/306712
Title:
Analytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS.
Authors:
Gross, Peter C; Burkart, Sonja C; Müller, Rolf
Abstract:
Purification and quality control of therapeutic peptides is often performed by one single method, RP-HPLC. As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel. The basic therapeutic peptide aviptadil served as a model substance to study the impurity profiles revealing 15 detectable impurities using CE-MS, two were detected by an appropriate nanoRP-HPLC-MS method. None of the impurities detected by CE were observed in LC and vice versa. The LOD in CE-MS was determined in the base peak electropherogram at ∼1fmol, a value 2500 times smaller than the LOD found in nanoRP-HPLC-MS (3pmol). In nanoRP-HPLC-MS only 0.2% of the extrapolated CE-MS signal for a 25ng aviptadil load was observed. We conclude that both, the LOD as well as the impurity profile of aviptadil, as analyzed by nanoRP-HPLC are influenced by both, the ligand-derivatized silica matrix and the flow-rate. Peptides may disappear completely and their variable emergence may lead to the determination of incorrect ratios as present in the sample.
Affiliation:
Biotech Processes and Analytics Department, PharmBioTec GmbH, D-66123 Saarbrücken, Germany. Electronic address: p.gross@pharmbiotec.de.
Citation:
Analytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS. 2014, 88:477-82 J Pharm Biomed Anal
Journal:
Journal of pharmaceutical and biomedical analysis
Issue Date:
Jan-2014
URI:
http://hdl.handle.net/10033/306712
DOI:
10.1016/j.jpba.2013.09.024
PubMed ID:
24176753
Type:
Article
Language:
en
ISSN:
1873-264X
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorGross, Peter Cen
dc.contributor.authorBurkart, Sonja Cen
dc.contributor.authorMüller, Rolfen
dc.date.accessioned2013-12-11T13:45:10Z-
dc.date.available2013-12-11T13:45:10Z-
dc.date.issued2014-01-
dc.identifier.citationAnalytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS. 2014, 88:477-82 J Pharm Biomed Analen
dc.identifier.issn1873-264X-
dc.identifier.pmid24176753-
dc.identifier.doi10.1016/j.jpba.2013.09.024-
dc.identifier.urihttp://hdl.handle.net/10033/306712-
dc.description.abstractPurification and quality control of therapeutic peptides is often performed by one single method, RP-HPLC. As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel. The basic therapeutic peptide aviptadil served as a model substance to study the impurity profiles revealing 15 detectable impurities using CE-MS, two were detected by an appropriate nanoRP-HPLC-MS method. None of the impurities detected by CE were observed in LC and vice versa. The LOD in CE-MS was determined in the base peak electropherogram at ∼1fmol, a value 2500 times smaller than the LOD found in nanoRP-HPLC-MS (3pmol). In nanoRP-HPLC-MS only 0.2% of the extrapolated CE-MS signal for a 25ng aviptadil load was observed. We conclude that both, the LOD as well as the impurity profile of aviptadil, as analyzed by nanoRP-HPLC are influenced by both, the ligand-derivatized silica matrix and the flow-rate. Peptides may disappear completely and their variable emergence may lead to the determination of incorrect ratios as present in the sample.en
dc.language.isoenen
dc.rightsArchived with thanks to Journal of pharmaceutical and biomedical analysisen
dc.titleAnalytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS.en
dc.typeArticleen
dc.contributor.departmentBiotech Processes and Analytics Department, PharmBioTec GmbH, D-66123 Saarbrücken, Germany. Electronic address: p.gross@pharmbiotec.de.en
dc.identifier.journalJournal of pharmaceutical and biomedical analysisen
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