Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children.

2.50
Hdl Handle:
http://hdl.handle.net/10033/311622
Title:
Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children.
Authors:
Gismondi, María Inés; Díaz Carrasco, Juan María; Valva, Pamela; Becker, Pablo Daniel; Guzmán, Carlos Alberto; Campos, Rodolfo Héctor; Preciado, María Victoria
Abstract:
Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2-9.6yr of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability. Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure.
Affiliation:
Helmholtz Centre for infection research, D-38124 Braunschweig, Germany
Citation:
Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children. 2013, 447 (1-2):187-96 Virology
Journal:
Virology
Issue Date:
Dec-2013
URI:
http://hdl.handle.net/10033/311622
DOI:
10.1016/j.virol.2013.09.002
PubMed ID:
24210114
Type:
Article
Language:
en
ISSN:
1096-0341
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGismondi, María Inésen
dc.contributor.authorDíaz Carrasco, Juan Maríaen
dc.contributor.authorValva, Pamelaen
dc.contributor.authorBecker, Pablo Danielen
dc.contributor.authorGuzmán, Carlos Albertoen
dc.contributor.authorCampos, Rodolfo Héctoren
dc.contributor.authorPreciado, María Victoriaen
dc.date.accessioned2014-01-20T14:17:27Z-
dc.date.available2014-01-20T14:17:27Z-
dc.date.issued2013-12-
dc.identifier.citationDynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children. 2013, 447 (1-2):187-96 Virologyen
dc.identifier.issn1096-0341-
dc.identifier.pmid24210114-
dc.identifier.doi10.1016/j.virol.2013.09.002-
dc.identifier.urihttp://hdl.handle.net/10033/311622-
dc.description.abstractClassic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2-9.6yr of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability. Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure.en
dc.language.isoenen
dc.rightsArchived with thanks to Virologyen
dc.subject.meshAdolescenten
dc.subject.meshBiotaen
dc.subject.meshChilden
dc.subject.meshChild, Preschoolen
dc.subject.meshCluster Analysisen
dc.subject.meshFemaleen
dc.subject.meshGenetic Variationen
dc.subject.meshHepacivirusen
dc.subject.meshHepatitis C, Chronicen
dc.subject.meshHumansen
dc.subject.meshLeukocytes, Mononuclearen
dc.subject.meshMaleen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPhylogenyen
dc.subject.meshPlasmaen
dc.subject.meshRNA, Viralen
dc.subject.meshRetrospective Studiesen
dc.subject.meshSequence Analysis, DNAen
dc.titleDynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, D-38124 Braunschweig, Germanyen
dc.identifier.journalVirologyen

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