Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm.

2.50
Hdl Handle:
http://hdl.handle.net/10033/317387
Title:
Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm.
Authors:
Vidigal, Pedrina G; Müsken, Mathias; Becker, Katrin A; Häussler, Susanne; Wingender, Jost; Steinmann, Eike; Kehrmann, Jan; Gulbins, Erich; Buer, Jan; Rath, Peter Michael; Steinmann, Jörg
Abstract:
We investigated the in vitro and in vivo activities of epigallocatechin-3-gallate (EGCg), a green tea component, against Stenotrophomonas maltophilia (Sm) isolates from cystic fibrosis (CF) patients. In vitro effects of EGCg and the antibiotic colistin (COL) on growth inhibition, survival, and also against young and mature biofilms of S. maltophilia were determined. Qualitative and quantitative changes on the biofilms were assessed by confocal laser scanning microscopy (CLSM). Further, in vivo effects of nebulized EGCg in C57BL/6 and Cftr mutant mice during acute Sm lung infection were evaluated. Subinhibitory concentrations of EGCg significantly reduced not only biofilm formation, but also the quantity of viable cells in young and mature biofilms. CLSM showed that EGCg-exposed biofilms exhibited either a change in total biofilm biovolume or an increase of the fraction of dead cells contained within the biofilm in a dose depended manner. Sm infected wild-type and Cftr mutant mice treated with 1,024 mg/L EGCg by inhalation exhibited significantly lower bacterial counts than those undergoing no treatment or treated with COL. EGCg displayed promising inhibitory and anti-biofilm properties against CF Sm isolates in vitro and significantly reduced Sm bacterial counts in an acute infection model with wild type and CF mice. This natural compound may represent a novel therapeutic agent against Sm infection in CF.
Affiliation:
Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany
Citation:
Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm. 2014, 9 (4):e92876 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/317387
DOI:
10.1371/journal.pone.0092876
PubMed ID:
24690894
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorVidigal, Pedrina Gen
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorBecker, Katrin Aen
dc.contributor.authorHäussler, Susanneen
dc.contributor.authorWingender, Josten
dc.contributor.authorSteinmann, Eikeen
dc.contributor.authorKehrmann, Janen
dc.contributor.authorGulbins, Erichen
dc.contributor.authorBuer, Janen
dc.contributor.authorRath, Peter Michaelen
dc.contributor.authorSteinmann, Jörgen
dc.date.accessioned2014-05-23T13:53:16Z-
dc.date.available2014-05-23T13:53:16Z-
dc.date.issued2014-
dc.identifier.citationEffects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm. 2014, 9 (4):e92876 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid24690894-
dc.identifier.doi10.1371/journal.pone.0092876-
dc.identifier.urihttp://hdl.handle.net/10033/317387-
dc.description.abstractWe investigated the in vitro and in vivo activities of epigallocatechin-3-gallate (EGCg), a green tea component, against Stenotrophomonas maltophilia (Sm) isolates from cystic fibrosis (CF) patients. In vitro effects of EGCg and the antibiotic colistin (COL) on growth inhibition, survival, and also against young and mature biofilms of S. maltophilia were determined. Qualitative and quantitative changes on the biofilms were assessed by confocal laser scanning microscopy (CLSM). Further, in vivo effects of nebulized EGCg in C57BL/6 and Cftr mutant mice during acute Sm lung infection were evaluated. Subinhibitory concentrations of EGCg significantly reduced not only biofilm formation, but also the quantity of viable cells in young and mature biofilms. CLSM showed that EGCg-exposed biofilms exhibited either a change in total biofilm biovolume or an increase of the fraction of dead cells contained within the biofilm in a dose depended manner. Sm infected wild-type and Cftr mutant mice treated with 1,024 mg/L EGCg by inhalation exhibited significantly lower bacterial counts than those undergoing no treatment or treated with COL. EGCg displayed promising inhibitory and anti-biofilm properties against CF Sm isolates in vitro and significantly reduced Sm bacterial counts in an acute infection model with wild type and CF mice. This natural compound may represent a novel therapeutic agent against Sm infection in CF.en
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen
dc.titleEffects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germanyen
dc.identifier.journalPloS oneen

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