Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany.

2.50
Hdl Handle:
http://hdl.handle.net/10033/320811
Title:
Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany.
Authors:
Henne, Karsten; Kahlisch, Leila; Brettar, Ingrid; Höfle, Manfred G
Abstract:
The bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly of Alphaproteobacteria (26% of all phylotypes), Gammaproteobacteria (11%), candidate division TM6 (11%), Chlamydiales (9%), and Betaproteobacteria (9%). The bulk water community consisted primarily of Bacteroidetes (25%), Betaproteobacteria (20%), Actinobacteria (16%), and Alphaproteobacteria (11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity.
Citation:
Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany. 2012, 78 (10):3530-8 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
May-2012
URI:
http://hdl.handle.net/10033/320811
DOI:
10.1128/AEM.06373-11
PubMed ID:
22389373
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorHenne, Karstenen
dc.contributor.authorKahlisch, Leilaen
dc.contributor.authorBrettar, Ingriden
dc.contributor.authorHöfle, Manfred Gen
dc.date.accessioned2014-06-11T14:30:02Z-
dc.date.available2014-06-11T14:30:02Z-
dc.date.issued2012-05-
dc.identifier.citationAnalysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany. 2012, 78 (10):3530-8 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336-
dc.identifier.pmid22389373-
dc.identifier.doi10.1128/AEM.06373-11-
dc.identifier.urihttp://hdl.handle.net/10033/320811-
dc.description.abstractThe bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly of Alphaproteobacteria (26% of all phylotypes), Gammaproteobacteria (11%), candidate division TM6 (11%), Chlamydiales (9%), and Betaproteobacteria (9%). The bulk water community consisted primarily of Bacteroidetes (25%), Betaproteobacteria (20%), Actinobacteria (16%), and Alphaproteobacteria (11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity.en
dc.language.isoenen
dc.rightsArchived with thanks to Applied and environmental microbiologyen
dc.subject.meshBacteriaen
dc.subject.meshBacterial Physiological Phenomenaen
dc.subject.meshBiofilmsen
dc.subject.meshBiotaen
dc.subject.meshCluster Analysisen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshDrinking Wateren
dc.subject.meshGermanyen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPhylogenyen
dc.subject.meshPolymorphism, Single-Stranded Conformationalen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSequence Analysis, DNAen
dc.titleAnalysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany.en
dc.typeArticleen
dc.identifier.journalApplied and environmental microbiologyen

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