2.50
Hdl Handle:
http://hdl.handle.net/10033/323434
Title:
Juxtanodin is an intrinsically disordered F-actin-binding protein.
Authors:
Ruskamo, Salla; Chukhlieb, Maryna; Vahokoski, Juha; Bhargav, Saligram Prabhakar; Liang, Fengyi; Kursula, Inari ( 0000-0001-5236-7056 ) ; Kursula, Petri ( 0000-0001-8529-3751 )
Abstract:
Juxtanodin, also called ermin, is an F-actin-binding protein expressed by oligodendrocytes, the myelin-forming cells of the central nervous system. While juxtanodin carries a short conserved F-actin-binding segment at its C terminus, it otherwise shares no similarity with known protein sequences. We carried out a structural characterization of recombinant juxtanodin in solution. Juxtanodin turned out to be intrinsically disordered, as evidenced by conventional and synchrotron radiation CD spectroscopy. Small-angle X-ray scattering indicated that juxtanodin is a monomeric, highly elongated, unfolded molecule. Ensemble optimization analysis of the data suggested also the presence of more compact forms of juxtanodin. The C terminus was a strict requirement for co-sedimentation of juxtanodin with microfilaments, but juxtanodin had only mild effects on actin polymerization. The disordered nature of juxtanodin may predict functions as a protein interaction hub, although F-actin is its only currently known binding partner.
Citation:
Juxtanodin is an intrinsically disordered F-actin-binding protein. 2012, 2:899 Sci Rep
Journal:
Scientific reports
Issue Date:
2012
URI:
http://hdl.handle.net/10033/323434
DOI:
10.1038/srep00899
PubMed ID:
23198089
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group CSSB

Full metadata record

DC FieldValue Language
dc.contributor.authorRuskamo, Sallaen
dc.contributor.authorChukhlieb, Marynaen
dc.contributor.authorVahokoski, Juhaen
dc.contributor.authorBhargav, Saligram Prabhakaren
dc.contributor.authorLiang, Fengyien
dc.contributor.authorKursula, Inarien
dc.contributor.authorKursula, Petrien
dc.date.accessioned2014-07-18T14:30:54Zen
dc.date.available2014-07-18T14:30:54Zen
dc.date.issued2012en
dc.identifier.citationJuxtanodin is an intrinsically disordered F-actin-binding protein. 2012, 2:899 Sci Repen
dc.identifier.issn2045-2322en
dc.identifier.pmid23198089en
dc.identifier.doi10.1038/srep00899en
dc.identifier.urihttp://hdl.handle.net/10033/323434en
dc.description.abstractJuxtanodin, also called ermin, is an F-actin-binding protein expressed by oligodendrocytes, the myelin-forming cells of the central nervous system. While juxtanodin carries a short conserved F-actin-binding segment at its C terminus, it otherwise shares no similarity with known protein sequences. We carried out a structural characterization of recombinant juxtanodin in solution. Juxtanodin turned out to be intrinsically disordered, as evidenced by conventional and synchrotron radiation CD spectroscopy. Small-angle X-ray scattering indicated that juxtanodin is a monomeric, highly elongated, unfolded molecule. Ensemble optimization analysis of the data suggested also the presence of more compact forms of juxtanodin. The C terminus was a strict requirement for co-sedimentation of juxtanodin with microfilaments, but juxtanodin had only mild effects on actin polymerization. The disordered nature of juxtanodin may predict functions as a protein interaction hub, although F-actin is its only currently known binding partner.en
dc.language.isoenen
dc.rightsArchived with thanks to Scientific reportsen
dc.subject.meshActinsen
dc.subject.meshAlgorithmsen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshAnimalsen
dc.subject.meshCarrier Proteinsen
dc.subject.meshCircular Dichroismen
dc.subject.meshMicrofilament Proteinsen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMuscle, Skeletalen
dc.subject.meshProtein Bindingen
dc.subject.meshProtein Structure, Secondaryen
dc.subject.meshRatsen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshScattering, Small Angleen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshSoftwareen
dc.subject.meshSolutionsen
dc.subject.meshSwineen
dc.subject.meshX-Ray Diffractionen
dc.titleJuxtanodin is an intrinsically disordered F-actin-binding protein.en
dc.typeArticleen
dc.identifier.journalScientific reportsen

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