In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response.

2.50
Hdl Handle:
http://hdl.handle.net/10033/323907
Title:
In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response.
Authors:
Ucciferri, Nadia; Collnot, Eva-Marie; Gaiser, Birgit K; Tirella, Annalisa; Stone, Vicki; Domenici, Claudio; Lehr, Claus-Michael; Ahluwalia, Arti
Abstract:
After passage through biological barriers, nanomaterials inevitably end up in contact with the vascular endothelium and can induce cardiovascular damage. In this study the toxicity and sub-lethal effects of six types of nanoparticle, including four of industrial and biomedical importance, on human endothelial cells were investigated using different in vitro assays. The results show that all the particles investigated induce some level of damage to the cells and that silver particles were most toxic, followed by titanium dioxide. Furthermore, endothelial cells were shown to be more susceptible when exposed to silver nanoparticles under flow conditions in a bioreactor. The study underlines that although simple in vitro tests are useful to screen compounds and to identify the type of effect induced on cells, they may not be sufficient to define safe exposure limits. Therefore, once initial toxicity screening has been conducted on nanomaterials, it is necessary to develop more physiologically relevant in vitro models to better understand how nanomaterials can impact on human health.
Citation:
In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response. 2014, 8 (6):697-708 Nanotoxicology
Journal:
Nanotoxicology
Issue Date:
Sep-2014
URI:
http://hdl.handle.net/10033/323907
DOI:
10.3109/17435390.2013.831500
PubMed ID:
23909703
Type:
Article
Language:
en
ISSN:
1743-5404
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorUcciferri, Nadiaen
dc.contributor.authorCollnot, Eva-Marieen
dc.contributor.authorGaiser, Birgit Ken
dc.contributor.authorTirella, Annalisaen
dc.contributor.authorStone, Vickien
dc.contributor.authorDomenici, Claudioen
dc.contributor.authorLehr, Claus-Michaelen
dc.contributor.authorAhluwalia, Artien
dc.date.accessioned2014-07-29T11:42:38Zen
dc.date.available2014-07-29T11:42:38Zen
dc.date.issued2014-09en
dc.identifier.citationIn vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response. 2014, 8 (6):697-708 Nanotoxicologyen
dc.identifier.issn1743-5404en
dc.identifier.pmid23909703en
dc.identifier.doi10.3109/17435390.2013.831500en
dc.identifier.urihttp://hdl.handle.net/10033/323907en
dc.description.abstractAfter passage through biological barriers, nanomaterials inevitably end up in contact with the vascular endothelium and can induce cardiovascular damage. In this study the toxicity and sub-lethal effects of six types of nanoparticle, including four of industrial and biomedical importance, on human endothelial cells were investigated using different in vitro assays. The results show that all the particles investigated induce some level of damage to the cells and that silver particles were most toxic, followed by titanium dioxide. Furthermore, endothelial cells were shown to be more susceptible when exposed to silver nanoparticles under flow conditions in a bioreactor. The study underlines that although simple in vitro tests are useful to screen compounds and to identify the type of effect induced on cells, they may not be sufficient to define safe exposure limits. Therefore, once initial toxicity screening has been conducted on nanomaterials, it is necessary to develop more physiologically relevant in vitro models to better understand how nanomaterials can impact on human health.en
dc.language.isoenen
dc.rightsArchived with thanks to Nanotoxicologyen
dc.subject.meshApoptosisen
dc.subject.meshCell Survivalen
dc.subject.meshCells, Cultureden
dc.subject.meshCytokinesen
dc.subject.meshDose-Response Relationship, Drugen
dc.subject.meshHuman Umbilical Vein Endothelial Cellsen
dc.subject.meshHumansen
dc.subject.meshNanoparticlesen
dc.subject.meshPolystyrenesen
dc.subject.meshSilveren
dc.subject.meshTitaniumen
dc.subject.meshvon Willebrand Factoren
dc.titleIn vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response.en
dc.typeArticleen
dc.identifier.journalNanotoxicologyen

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