2.50
Hdl Handle:
http://hdl.handle.net/10033/326194
Title:
SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors.
Authors:
Horbal, Liliya; Rebets, Yuriy; Rabyk, Mariya; Makitrynskyy, Roman; Luzhetskyy, Andriy ( 0000-0001-6497-0047 ) ; Fedorenko, Victor; Bechthold, Andreas
Abstract:
Analysis of the simocyclinone biosynthesis (sim) gene cluster of Streptomyces antibioticus Tü6040 led to the identification of a putative pathway specific regulatory gene simReg1. In silico analysis places the SimReg1 protein in the OmpR-PhoB subfamily of response regulators. Gene replacement of simReg1 from the S. antibioticus chromosome completely abolishes simocyclinone production indicating that SimReg1 is a key regulator of simocyclinone biosynthesis. Results of the DNA-shift assays and reporter gene expression analysis are consistent with the idea that SimReg1 activates transcription of simocyclinone biosynthesis, transporter genes, regulatory gene simReg3 and his own transcription. The presence of extracts (simocyclinone) from S. antibioticus Tü6040 × pSSimR1-1 could dissociate SimReg1 from promoter regions. A preliminary model for regulation of simocyclinone biosynthesis and export is discussed.
Citation:
SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors. 2012, 2 (1):1 AMB Express
Journal:
AMB Express
Issue Date:
2012
URI:
http://hdl.handle.net/10033/326194
DOI:
10.1186/2191-0855-2-1
PubMed ID:
22214346
Type:
Article
Language:
en
ISSN:
2191-0855
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorHorbal, Liliyaen
dc.contributor.authorRebets, Yuriyen
dc.contributor.authorRabyk, Mariyaen
dc.contributor.authorMakitrynskyy, Romanen
dc.contributor.authorLuzhetskyy, Andriyen
dc.contributor.authorFedorenko, Victoren
dc.contributor.authorBechthold, Andreasen
dc.date.accessioned2014-09-17T11:36:55Zen
dc.date.available2014-09-17T11:36:55Zen
dc.date.issued2012en
dc.identifier.citationSimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors. 2012, 2 (1):1 AMB Expressen
dc.identifier.issn2191-0855en
dc.identifier.pmid22214346en
dc.identifier.doi10.1186/2191-0855-2-1en
dc.identifier.urihttp://hdl.handle.net/10033/326194en
dc.description.abstractAnalysis of the simocyclinone biosynthesis (sim) gene cluster of Streptomyces antibioticus Tü6040 led to the identification of a putative pathway specific regulatory gene simReg1. In silico analysis places the SimReg1 protein in the OmpR-PhoB subfamily of response regulators. Gene replacement of simReg1 from the S. antibioticus chromosome completely abolishes simocyclinone production indicating that SimReg1 is a key regulator of simocyclinone biosynthesis. Results of the DNA-shift assays and reporter gene expression analysis are consistent with the idea that SimReg1 activates transcription of simocyclinone biosynthesis, transporter genes, regulatory gene simReg3 and his own transcription. The presence of extracts (simocyclinone) from S. antibioticus Tü6040 × pSSimR1-1 could dissociate SimReg1 from promoter regions. A preliminary model for regulation of simocyclinone biosynthesis and export is discussed.en
dc.language.isoenen
dc.rightsArchived with thanks to AMB Expressen
dc.titleSimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors.en
dc.typeArticleen
dc.identifier.journalAMB Expressen

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