2.50
Hdl Handle:
http://hdl.handle.net/10033/332642
Title:
Experimental selection of long-term intracellular mycobacteria.
Authors:
Vázquez, Cristina L; Lerner, Thomas R; Kasmapour, Bahram; Pei, Gang; Gronow, Achim; Bianco, Maria V; Blanco, Federico C; Bleck, Christopher K E ( 0000-0003-4409-016X ) ; Geffers, Robert ( 0000-0003-4409-016X ) ; Bigi, Fabiana; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Gutierrez, Maximiliano G
Abstract:
Some intracellular bacteria are known to cause long-term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long-term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re-infection and also with the specific expression of stress- and survival-related genes. Our findings identify bacterial traits implicated in the establishment of long-term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
Citation:
Experimental selection of long-term intracellular mycobacteria. 2014, 16 (9):1425-40 Cell. Microbiol.
Journal:
Cellular microbiology
Issue Date:
Sep-2014
URI:
http://hdl.handle.net/10033/332642
DOI:
10.1111/cmi.12303
PubMed ID:
24779357
Type:
Article
Language:
en
ISSN:
1462-5822
Appears in Collections:
Publications of the RG Phagosomen Biologie

Full metadata record

DC FieldValue Language
dc.contributor.authorVázquez, Cristina Len
dc.contributor.authorLerner, Thomas Ren
dc.contributor.authorKasmapour, Bahramen
dc.contributor.authorPei, Gangen
dc.contributor.authorGronow, Achimen
dc.contributor.authorBianco, Maria Ven
dc.contributor.authorBlanco, Federico Cen
dc.contributor.authorBleck, Christopher K Een
dc.contributor.authorGeffers, Roberten
dc.contributor.authorBigi, Fabianaen
dc.contributor.authorAbraham, Wolf-Raineren
dc.contributor.authorGutierrez, Maximiliano Gen
dc.date.accessioned2014-10-10T08:43:59Zen
dc.date.available2014-10-10T08:43:59Zen
dc.date.issued2014-09en
dc.identifier.citationExperimental selection of long-term intracellular mycobacteria. 2014, 16 (9):1425-40 Cell. Microbiol.en
dc.identifier.issn1462-5822en
dc.identifier.pmid24779357en
dc.identifier.doi10.1111/cmi.12303en
dc.identifier.urihttp://hdl.handle.net/10033/332642en
dc.description.abstractSome intracellular bacteria are known to cause long-term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long-term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re-infection and also with the specific expression of stress- and survival-related genes. Our findings identify bacterial traits implicated in the establishment of long-term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.en
dc.language.isoenen
dc.rightsArchived with thanks to Cellular microbiologyen
dc.titleExperimental selection of long-term intracellular mycobacteria.en
dc.typeArticleen
dc.identifier.journalCellular microbiologyen

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