In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses.

2.50
Hdl Handle:
http://hdl.handle.net/10033/332645
Title:
In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses.
Authors:
Regus-Leidig, Hanna; Fuchs, Michaela; Löhner, Martina; Leist, Sarah R; Leal-Ortiz, Sergio; Chiodo, Vince A; Hauswirth, William W; Garner, Craig C; Brandstätter, Johann H
Abstract:
Piccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure.
Citation:
In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses. 2014, 8:259 Front Cell Neurosci
Journal:
Frontiers in cellular neuroscience
Issue Date:
2014
URI:
http://hdl.handle.net/10033/332645
DOI:
10.3389/fncel.2014.00259
PubMed ID:
25232303
Type:
Article
Language:
en
ISSN:
1662-5102
Appears in Collections:
publications of the department infection genetics (INFG)

Full metadata record

DC FieldValue Language
dc.contributor.authorRegus-Leidig, Hannaen
dc.contributor.authorFuchs, Michaelaen
dc.contributor.authorLöhner, Martinaen
dc.contributor.authorLeist, Sarah Ren
dc.contributor.authorLeal-Ortiz, Sergioen
dc.contributor.authorChiodo, Vince Aen
dc.contributor.authorHauswirth, William Wen
dc.contributor.authorGarner, Craig Cen
dc.contributor.authorBrandstätter, Johann Hen
dc.date.accessioned2014-10-10T09:40:19Z-
dc.date.available2014-10-10T09:40:19Z-
dc.date.issued2014-
dc.identifier.citationIn vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses. 2014, 8:259 Front Cell Neuroscien
dc.identifier.issn1662-5102-
dc.identifier.pmid25232303-
dc.identifier.doi10.3389/fncel.2014.00259-
dc.identifier.urihttp://hdl.handle.net/10033/332645-
dc.description.abstractPiccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure.en
dc.language.isoenen
dc.rightsArchived with thanks to Frontiers in cellular neuroscienceen
dc.titleIn vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses.en
dc.typeArticleen
dc.identifier.journalFrontiers in cellular neuroscienceen

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