2.50
Hdl Handle:
http://hdl.handle.net/10033/332725
Title:
Localization of MLH3 at the centrosomes.
Authors:
Roesner, Lennart M; Mielke, Christian; Faehnrich, Silke; Merkhoffer, Yvonne; Dittmar, Kurt E J; Drexler, Hans G; Dirks, Wilhelm G
Abstract:
Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.
Citation:
Localization of MLH3 at the centrosomes. 2014, 15 (8):13932-7 Int J Mol Sci
Journal:
International journal of molecular sciences
Issue Date:
2014
URI:
http://hdl.handle.net/10033/332725
DOI:
10.3390/ijms150813932
PubMed ID:
25116689
Type:
Article
Language:
en
ISSN:
1422-0067
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorRoesner, Lennart Men
dc.contributor.authorMielke, Christianen
dc.contributor.authorFaehnrich, Silkeen
dc.contributor.authorMerkhoffer, Yvonneen
dc.contributor.authorDittmar, Kurt E Jen
dc.contributor.authorDrexler, Hans Gen
dc.contributor.authorDirks, Wilhelm Gen
dc.date.accessioned2014-10-13T14:10:38Z-
dc.date.available2014-10-13T14:10:38Z-
dc.date.issued2014-
dc.identifier.citationLocalization of MLH3 at the centrosomes. 2014, 15 (8):13932-7 Int J Mol Scien
dc.identifier.issn1422-0067-
dc.identifier.pmid25116689-
dc.identifier.doi10.3390/ijms150813932-
dc.identifier.urihttp://hdl.handle.net/10033/332725-
dc.description.abstractMutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.en
dc.language.isoenen
dc.rightsArchived with thanks to International journal of molecular sciencesen
dc.titleLocalization of MLH3 at the centrosomes.en
dc.typeArticleen
dc.identifier.journalInternational journal of molecular sciencesen

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