2.50
Hdl Handle:
http://hdl.handle.net/10033/332841
Title:
Cyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice.
Authors:
Skrnjug, Ivana; Guzmán, Carlos Alberto; Ruecker, Christine
Abstract:
The recently discovered mammalian enzyme cyclic GMP-AMP synthase produces cyclic GMP-AMP (cGAMP) after being activated by pathogen-derived cytosolic double stranded DNA. The product can stimulate STING-dependent interferon type I signaling. Here, we explore the efficacy of cGAMP as a mucosal adjuvant in mice. We show that cGAMP can enhance the adaptive immune response to the model antigen ovalbumin. It promotes antigen specific IgG and a balanced Th1/Th2 lymphocyte response in immunized mice. A characteristic of the cGAMP-induced immune response is the slightly reduced induction of interleukin-17 as a hallmark of Th17 activity - a distinct feature that is not observed with other cyclic di-nucleotide adjuvants. We further characterize the innate immune stimulation activity in vitro on murine bone marrow-derived dendritic cells and human dendritic cells. The observed results suggest the consideration of cGAMP as a candidate mucosal adjuvant for human vaccines.
Affiliation:
Helmholtz Cente for infection research,Inhoffenstr. 7, D38124 Braunschweig, Germany.
Citation:
Cyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice. 2014, 9 (10):e110150 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/332841
DOI:
10.1371/journal.pone.0110150
PubMed ID:
25295996
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorSkrnjug, Ivanaen
dc.contributor.authorGuzmán, Carlos Albertoen
dc.contributor.authorRuecker, Christineen
dc.date.accessioned2014-10-17T08:20:49Z-
dc.date.available2014-10-17T08:20:49Z-
dc.date.issued2014-
dc.identifier.citationCyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice. 2014, 9 (10):e110150 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid25295996-
dc.identifier.doi10.1371/journal.pone.0110150-
dc.identifier.urihttp://hdl.handle.net/10033/332841-
dc.description.abstractThe recently discovered mammalian enzyme cyclic GMP-AMP synthase produces cyclic GMP-AMP (cGAMP) after being activated by pathogen-derived cytosolic double stranded DNA. The product can stimulate STING-dependent interferon type I signaling. Here, we explore the efficacy of cGAMP as a mucosal adjuvant in mice. We show that cGAMP can enhance the adaptive immune response to the model antigen ovalbumin. It promotes antigen specific IgG and a balanced Th1/Th2 lymphocyte response in immunized mice. A characteristic of the cGAMP-induced immune response is the slightly reduced induction of interleukin-17 as a hallmark of Th17 activity - a distinct feature that is not observed with other cyclic di-nucleotide adjuvants. We further characterize the innate immune stimulation activity in vitro on murine bone marrow-derived dendritic cells and human dendritic cells. The observed results suggest the consideration of cGAMP as a candidate mucosal adjuvant for human vaccines.en
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen
dc.titleCyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Cente for infection research,Inhoffenstr. 7, D38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.