A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC.

2.50
Hdl Handle:
http://hdl.handle.net/10033/333679
Title:
A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC.
Authors:
Nuss, Aaron M; Schuster, Franziska; Kathrin Heroven, Ann; Heine, Wiebke; Pisano, Fabio; Dersch, Petra ( 0000-0001-8177-3280 )
Abstract:
In this study we investigated the influence of the global response regulator PhoP on the complex regulatory cascade controlling expression of early stage virulence genes of Yersinia pseudotuberculosis via the virulence regulator RovA. Our analysis revealed the following novel features: (1) PhoP activates expression of the CsrC RNA in Y. pseudotuberculosis, leading to activation of RovA synthesis through the CsrABC-RovM cascade, (2) activation of csrC transcription is direct and PhoP is shown to bind to two separate PhoP box-like sites, (3) PhoP-mediated activation results in transcription from two different promoters closely downstream of the PhoP binding sites, leading to two distinct CsrC RNAs, and (4) the stability of the CsrC RNAs differs significantly between the Y. pseudotuberculosis strains YPIII and IP32953 due to a 20 nucleotides insertion in CsrC(IP32953), which renders the transcript more susceptible to degradation. In summary, our study showed that PhoP-mediated influence on the regulatory cascade controlling the Csr system and RovA in Y. pseudotuberculosis varies within the species, suggesting that the Csr system is a focal point to readjust and adapt the genus to different hosts and reservoirs.
Affiliation:
Department of Molecular Infection Biology; Helmholtz Centre for Infection Research; Braunschweig, Germany.
Citation:
A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC. 2014, 11 (5):580-93 RNA Biol
Journal:
RNA biology
Issue Date:
May-2014
URI:
http://hdl.handle.net/10033/333679
PubMed ID:
24786463
Type:
Article
Language:
en
ISSN:
1555-8584
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorSchuster, Franziskaen
dc.contributor.authorKathrin Heroven, Annen
dc.contributor.authorHeine, Wiebkeen
dc.contributor.authorPisano, Fabioen
dc.contributor.authorDersch, Petraen
dc.date.accessioned2014-11-04T11:05:40Zen
dc.date.available2014-11-04T11:05:40Zen
dc.date.issued2014-05en
dc.identifier.citationA direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC. 2014, 11 (5):580-93 RNA Biolen
dc.identifier.issn1555-8584en
dc.identifier.pmid24786463en
dc.identifier.urihttp://hdl.handle.net/10033/333679en
dc.description.abstractIn this study we investigated the influence of the global response regulator PhoP on the complex regulatory cascade controlling expression of early stage virulence genes of Yersinia pseudotuberculosis via the virulence regulator RovA. Our analysis revealed the following novel features: (1) PhoP activates expression of the CsrC RNA in Y. pseudotuberculosis, leading to activation of RovA synthesis through the CsrABC-RovM cascade, (2) activation of csrC transcription is direct and PhoP is shown to bind to two separate PhoP box-like sites, (3) PhoP-mediated activation results in transcription from two different promoters closely downstream of the PhoP binding sites, leading to two distinct CsrC RNAs, and (4) the stability of the CsrC RNAs differs significantly between the Y. pseudotuberculosis strains YPIII and IP32953 due to a 20 nucleotides insertion in CsrC(IP32953), which renders the transcript more susceptible to degradation. In summary, our study showed that PhoP-mediated influence on the regulatory cascade controlling the Csr system and RovA in Y. pseudotuberculosis varies within the species, suggesting that the Csr system is a focal point to readjust and adapt the genus to different hosts and reservoirs.en
dc.language.isoenen
dc.titleA direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Infection Biology; Helmholtz Centre for Infection Research; Braunschweig, Germany.en
dc.identifier.journalRNA biologyen
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