2.50
Hdl Handle:
http://hdl.handle.net/10033/333702
Title:
Periostin secreted by mesenchymal stem cells supports tendon formation in an ectopic mouse model.
Authors:
Noack, Sandra; Seiffart, Virginia; Willbold, Elmar; Laggies, Sandra; Winkel, Andreas; Shahab-Osterloh, Sandra; Flörkemeier, Thilo; Hertwig, Falk; Steinhoff, Christine; Nuber, Ulrike A; Gross, Gerhard; Hoffmann, Andrea
Abstract:
True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T½, overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L+MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert a major impact on tendon development. Gene expression analyses revealed four genes encoding secreted factors that are notably upregulated: periostin, C-type lectin domain family 3 (member b), RNase A4, and follistatin-like 1. These factors have not previously been implicated in tendon biology. Among these, periostin showed a specific expression in tenocytes of adult mouse Achilles tendon and in chondrocytes within the nonmineralized fibrocartilage zone of the enthesis with the calcaneus. Overexpression of periostin alone or in combination with constitutively active BMP receptor type in human mesenchymal stem cells and subsequent implantation into ectopic sites in mice demonstrated a reproducible moderate tenogenic capacity that has not been described before. Therefore, periostin may belong to the factors contributing to the development of tenogenic tissue.
Affiliation:
1 Department of Orthopaedic Trauma, Hannover Medical School (MHH), Hannover, Germany .
Citation:
Periostin secreted by mesenchymal stem cells supports tendon formation in an ectopic mouse model. 2014, 23 (16):1844-57 Stem Cells Dev.
Journal:
Stem cells and development
Issue Date:
15-Aug-2014
URI:
http://hdl.handle.net/10033/333702
DOI:
10.1089/scd.2014.0124
PubMed ID:
24809660
Type:
Article
Language:
en
ISSN:
1557-8534
Appears in Collections:
publications of the research group modell systems for infections and immunity (MSYS)

Full metadata record

DC FieldValue Language
dc.contributor.authorNoack, Sandraen
dc.contributor.authorSeiffart, Virginiaen
dc.contributor.authorWillbold, Elmaren
dc.contributor.authorLaggies, Sandraen
dc.contributor.authorWinkel, Andreasen
dc.contributor.authorShahab-Osterloh, Sandraen
dc.contributor.authorFlörkemeier, Thiloen
dc.contributor.authorHertwig, Falken
dc.contributor.authorSteinhoff, Christineen
dc.contributor.authorNuber, Ulrike Aen
dc.contributor.authorGross, Gerharden
dc.contributor.authorHoffmann, Andreaen
dc.date.accessioned2014-11-04T13:32:43Z-
dc.date.available2014-11-04T13:32:43Z-
dc.date.issued2014-08-15-
dc.identifier.citationPeriostin secreted by mesenchymal stem cells supports tendon formation in an ectopic mouse model. 2014, 23 (16):1844-57 Stem Cells Dev.en
dc.identifier.issn1557-8534-
dc.identifier.pmid24809660-
dc.identifier.doi10.1089/scd.2014.0124-
dc.identifier.urihttp://hdl.handle.net/10033/333702-
dc.description.abstractTrue tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T½, overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L+MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert a major impact on tendon development. Gene expression analyses revealed four genes encoding secreted factors that are notably upregulated: periostin, C-type lectin domain family 3 (member b), RNase A4, and follistatin-like 1. These factors have not previously been implicated in tendon biology. Among these, periostin showed a specific expression in tenocytes of adult mouse Achilles tendon and in chondrocytes within the nonmineralized fibrocartilage zone of the enthesis with the calcaneus. Overexpression of periostin alone or in combination with constitutively active BMP receptor type in human mesenchymal stem cells and subsequent implantation into ectopic sites in mice demonstrated a reproducible moderate tenogenic capacity that has not been described before. Therefore, periostin may belong to the factors contributing to the development of tenogenic tissue.en
dc.language.isoenen
dc.titlePeriostin secreted by mesenchymal stem cells supports tendon formation in an ectopic mouse model.en
dc.typeArticleen
dc.contributor.department1 Department of Orthopaedic Trauma, Hannover Medical School (MHH), Hannover, Germany .en
dc.identifier.journalStem cells and developmenten

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