2.50
Hdl Handle:
http://hdl.handle.net/10033/338197
Title:
The extensive set of accessory Pseudomonas aeruginosa genomic components.
Authors:
Pohl, Sarah; Klockgether, Jens; Eckweiler, Denitsa; Khaledi, Ariane; Schniederjans, Monika; Chouvarine, Philippe; Tümmler, Burkhard; Häussler, Susanne
Abstract:
Up to 20% of the chromosomal Pseudomonas aeruginosa DNA belong to the so-called accessory genome. Its elements are specific for subgroups or even single strains and are likely acquired by horizontal gene transfer (HGT). Similarities of the accessory genomic elements to DNA from other bacterial species, mainly the DNA of γ- and β-proteobacteria, indicate a role of interspecies HGT. In this study, we analysed the expression of the accessory genome in 150 clinical P. aeruginosa isolates as uncovered by transcriptome sequencing and the presence of accessory genes in eleven additional isolates. Remarkably, despite the large number of P. aeruginosa strains that have been sequenced to date, we found new strain-specific compositions of accessory genomic elements and a high portion (10-20%) of genes without P. aeruginosa homologues. Although some genes were detected to be expressed/present in several isolates, individual patterns regarding the genes, their functions and the possible origin of the DNA were widespread among the tested strains. Our results demonstrate the unaltered potential to discover new traits within the P. aeruginosa population and underline that the P. aeruginosa pangenome is likely to increase with increasing sequence information.
Citation:
The extensive set of accessory Pseudomonas aeruginosa genomic components. 2014, 356 (2):235-41 FEMS Microbiol. Lett.
Journal:
FEMS microbiology letters
Issue Date:
Jul-2014
URI:
http://hdl.handle.net/10033/338197
DOI:
10.1111/1574-6968.12445
PubMed ID:
24766399
Type:
Article
Language:
en
ISSN:
1574-6968
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorPohl, Sarahen
dc.contributor.authorKlockgether, Jensen
dc.contributor.authorEckweiler, Denitsaen
dc.contributor.authorKhaledi, Arianeen
dc.contributor.authorSchniederjans, Monikaen
dc.contributor.authorChouvarine, Philippeen
dc.contributor.authorTümmler, Burkharden
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2015-01-13T15:52:31Z-
dc.date.available2015-01-13T15:52:31Z-
dc.date.issued2014-07-
dc.identifier.citationThe extensive set of accessory Pseudomonas aeruginosa genomic components. 2014, 356 (2):235-41 FEMS Microbiol. Lett.en
dc.identifier.issn1574-6968-
dc.identifier.pmid24766399-
dc.identifier.doi10.1111/1574-6968.12445-
dc.identifier.urihttp://hdl.handle.net/10033/338197-
dc.description.abstractUp to 20% of the chromosomal Pseudomonas aeruginosa DNA belong to the so-called accessory genome. Its elements are specific for subgroups or even single strains and are likely acquired by horizontal gene transfer (HGT). Similarities of the accessory genomic elements to DNA from other bacterial species, mainly the DNA of γ- and β-proteobacteria, indicate a role of interspecies HGT. In this study, we analysed the expression of the accessory genome in 150 clinical P. aeruginosa isolates as uncovered by transcriptome sequencing and the presence of accessory genes in eleven additional isolates. Remarkably, despite the large number of P. aeruginosa strains that have been sequenced to date, we found new strain-specific compositions of accessory genomic elements and a high portion (10-20%) of genes without P. aeruginosa homologues. Although some genes were detected to be expressed/present in several isolates, individual patterns regarding the genes, their functions and the possible origin of the DNA were widespread among the tested strains. Our results demonstrate the unaltered potential to discover new traits within the P. aeruginosa population and underline that the P. aeruginosa pangenome is likely to increase with increasing sequence information.en
dc.language.isoenen
dc.relationeu-repo/grantAgreement/EC/FP7/260276en
dc.rightsembargoedAccessen
dc.titleThe extensive set of accessory Pseudomonas aeruginosa genomic components.en
dc.typeArticleen
dc.identifier.journalFEMS microbiology lettersen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.