Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.

2.50
Hdl Handle:
http://hdl.handle.net/10033/338491
Title:
Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.
Authors:
Blanka, Andrea; Schulz, Sebastian; Eckweiler, Denitsa; Franke, Raimo; Bielecka, Agata; Nicolai, Tanja; Casilag, Fiordiligie; Düvel, Juliane; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Kaever, Volkhard; Häussler, Susanne ( 0000-0001-6141-9102 )
Abstract:
Pseudomonas aeruginosa is distinguished by its broad metabolic diversity and its remarkable capability for adaptation, which relies on a large collection of transcriptional regulators and alternative sigma (σ) factors. The largest group of alternative σ factors is that of the extracytoplasmic function (ECF) σ factors, which control key transduction pathways for maintenance of envelope homeostasis in response to external stress and cell growth. In addition, there are specific roles of alternative σ factors in regulating the expression of virulence and virulence-associated genes. Here, we analyzed a deletion mutant of the ECF σ factor SigX and applied mRNA profiling to define the SigX-dependent regulon in P. aeruginosa in response to low-osmolarity-medium conditions. Furthermore, the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) led to the identification of the DNA binding motif of SigX. Genome-wide mapping of SigX-binding regions revealed enrichment of downstream genes involved in fatty acid biosynthesis, type III secretion, swarming and cyclic di-GMP (c-di-GMP) signaling. In accordance, a sigX deletion mutant exhibited altered fatty acid composition of the cell membrane, reduced cytotoxicity, impaired swarming activity, elevated c-di-GMP levels, and increased biofilm formation. In conclusion, a combination of ChIP-seq with transcriptional profiling and bioinformatic approaches to define consensus DNA binding sequences proved to be effective for the elucidation of the regulon of the alternative σ factor SigX, revealing its role in complex virulence-associated phenotypes in P. aeruginosa.
Affiliation:
Helmholtz Centre of infection research; Inhoffenstr. 7; D38124 Braunschweig; Germany.
Citation:
Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity. 2014, 196 (2):345-56 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Jan-2014
URI:
http://hdl.handle.net/10033/338491
DOI:
10.1128/JB.01034-13
PubMed ID:
24187091
Type:
Article
Language:
en
ISSN:
1098-5530
Appears in Collections:
openaire; publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorBlanka, Andreaen
dc.contributor.authorSchulz, Sebastianen
dc.contributor.authorEckweiler, Denitsaen
dc.contributor.authorFranke, Raimoen
dc.contributor.authorBielecka, Agataen
dc.contributor.authorNicolai, Tanjaen
dc.contributor.authorCasilag, Fiordiligieen
dc.contributor.authorDüvel, Julianeen
dc.contributor.authorAbraham, Wolf-Raineren
dc.contributor.authorKaever, Volkharden
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2015-01-16T15:07:52Z-
dc.date.available2015-01-16T15:07:52Z-
dc.date.issued2014-01-
dc.identifier.citationIdentification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity. 2014, 196 (2):345-56 J. Bacteriol.en
dc.identifier.issn1098-5530-
dc.identifier.pmid24187091-
dc.identifier.doi10.1128/JB.01034-13-
dc.identifier.urihttp://hdl.handle.net/10033/338491-
dc.description.abstractPseudomonas aeruginosa is distinguished by its broad metabolic diversity and its remarkable capability for adaptation, which relies on a large collection of transcriptional regulators and alternative sigma (σ) factors. The largest group of alternative σ factors is that of the extracytoplasmic function (ECF) σ factors, which control key transduction pathways for maintenance of envelope homeostasis in response to external stress and cell growth. In addition, there are specific roles of alternative σ factors in regulating the expression of virulence and virulence-associated genes. Here, we analyzed a deletion mutant of the ECF σ factor SigX and applied mRNA profiling to define the SigX-dependent regulon in P. aeruginosa in response to low-osmolarity-medium conditions. Furthermore, the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) led to the identification of the DNA binding motif of SigX. Genome-wide mapping of SigX-binding regions revealed enrichment of downstream genes involved in fatty acid biosynthesis, type III secretion, swarming and cyclic di-GMP (c-di-GMP) signaling. In accordance, a sigX deletion mutant exhibited altered fatty acid composition of the cell membrane, reduced cytotoxicity, impaired swarming activity, elevated c-di-GMP levels, and increased biofilm formation. In conclusion, a combination of ChIP-seq with transcriptional profiling and bioinformatic approaches to define consensus DNA binding sequences proved to be effective for the elucidation of the regulon of the alternative σ factor SigX, revealing its role in complex virulence-associated phenotypes in P. aeruginosa.en
dc.language.isoenen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/260276/en
dc.rightsopenAccessen
dc.subject.meshBinding Sitesen
dc.subject.meshChromatin Immunoprecipitationen
dc.subject.meshCulture Mediaen
dc.subject.meshGene Deletionen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshHigh-Throughput Nucleotide Sequencingen
dc.subject.meshOsmotic Pressureen
dc.subject.meshProtein Bindingen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshRegulonen
dc.subject.meshSigma Factoren
dc.subject.meshVirulence Factorsen
dc.titleIdentification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre of infection research; Inhoffenstr. 7; D38124 Braunschweig; Germany.en
dc.identifier.journalJournal of bacteriologyen

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