The EHEC-host interactome reveals novel targets for the translocated intimin receptor.

2.50
Hdl Handle:
http://hdl.handle.net/10033/338555
Title:
The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
Authors:
Blasche, Sonja; Arens, Stefan; Ceol, Arnaud; Siszler, Gabriella; Schmidt, M Alexander; Häuser, Roman; Schwarz, Frank; Wuchty, Stefan; Aloy, Patrick; Uetz, Peter; Stradal, Theresia; Koegl, Manfred
Abstract:
Enterohemorrhagic E. coli (EHEC) manipulate their human host through at least 39 effector proteins which hijack host processes through direct protein-protein interactions (PPIs). To identify their protein targets in the host cells, we performed yeast two-hybrid screens, allowing us to find 48 high-confidence protein-protein interactions between 15 EHEC effectors and 47 human host proteins. In comparison to other bacteria and viruses we found that EHEC effectors bind more frequently to hub proteins as well as to proteins that participate in a higher number of protein complexes. The data set includes six new interactions that involve the translocated intimin receptor (TIR), namely HPCAL1, HPCAL4, NCALD, ARRB1, PDE6D, and STK16. We compared these TIR interactions in EHEC and enteropathogenic E. coli (EPEC) and found that five interactions were conserved. Notably, the conserved interactions included those of serine/threonine kinase 16 (STK16), hippocalcin-like 1 (HPCAL1) as well as neurocalcin-delta (NCALD). These proteins co-localize with the infection sites of EPEC. Furthermore, our results suggest putative functions of poorly characterized effectors (EspJ, EspY1). In particular, we observed that EspJ is connected to the microtubule system while EspY1 appears to be involved in apoptosis/cell cycle regulation.
Affiliation:
Helmholtz Centre for infection research; Inhooffenstr. 7; D-38124 Braunschweig; Germany.
Citation:
The EHEC-host interactome reveals novel targets for the translocated intimin receptor. 2014, 4:7531 Sci Rep
Journal:
Scientific reports
Issue Date:
2014
URI:
http://hdl.handle.net/10033/338555
DOI:
10.1038/srep07531
PubMed ID:
25519916
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the department of cell biology (ZBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorBlasche, Sonjaen
dc.contributor.authorArens, Stefanen
dc.contributor.authorCeol, Arnauden
dc.contributor.authorSiszler, Gabriellaen
dc.contributor.authorSchmidt, M Alexanderen
dc.contributor.authorHäuser, Romanen
dc.contributor.authorSchwarz, Franken
dc.contributor.authorWuchty, Stefanen
dc.contributor.authorAloy, Patricken
dc.contributor.authorUetz, Peteren
dc.contributor.authorStradal, Theresiaen
dc.contributor.authorKoegl, Manfreden
dc.date.accessioned2015-01-19T15:02:33Z-
dc.date.available2015-01-19T15:02:33Z-
dc.date.issued2014-
dc.identifier.citationThe EHEC-host interactome reveals novel targets for the translocated intimin receptor. 2014, 4:7531 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid25519916-
dc.identifier.doi10.1038/srep07531-
dc.identifier.urihttp://hdl.handle.net/10033/338555-
dc.description.abstractEnterohemorrhagic E. coli (EHEC) manipulate their human host through at least 39 effector proteins which hijack host processes through direct protein-protein interactions (PPIs). To identify their protein targets in the host cells, we performed yeast two-hybrid screens, allowing us to find 48 high-confidence protein-protein interactions between 15 EHEC effectors and 47 human host proteins. In comparison to other bacteria and viruses we found that EHEC effectors bind more frequently to hub proteins as well as to proteins that participate in a higher number of protein complexes. The data set includes six new interactions that involve the translocated intimin receptor (TIR), namely HPCAL1, HPCAL4, NCALD, ARRB1, PDE6D, and STK16. We compared these TIR interactions in EHEC and enteropathogenic E. coli (EPEC) and found that five interactions were conserved. Notably, the conserved interactions included those of serine/threonine kinase 16 (STK16), hippocalcin-like 1 (HPCAL1) as well as neurocalcin-delta (NCALD). These proteins co-localize with the infection sites of EPEC. Furthermore, our results suggest putative functions of poorly characterized effectors (EspJ, EspY1). In particular, we observed that EspJ is connected to the microtubule system while EspY1 appears to be involved in apoptosis/cell cycle regulation.en
dc.language.isoenen
dc.titleThe EHEC-host interactome reveals novel targets for the translocated intimin receptor.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research; Inhooffenstr. 7; D-38124 Braunschweig; Germany.en
dc.identifier.journalScientific reportsen

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