Advantages of Foxp3(+) regulatory T cell depletion using DEREG mice.

2.50
Hdl Handle:
http://hdl.handle.net/10033/338558
Title:
Advantages of Foxp3(+) regulatory T cell depletion using DEREG mice.
Authors:
Mayer, Christian T; Lahl, Katharina; Milanez-Almeida, Pedro; Watts, Deepika; Dittmer, Ulf; Fyhrquist, Nanna; Huehn, Jochen; Kopf, Manfred; Kretschmer, Karsten; Rouse, Barry; Sparwasser, Tim ( 0000-0001-5645-902X )
Abstract:
Several mechanisms enable immunological self-tolerance. Regulatory T cells (Tregs) are a specialized T cell subset that prevents autoimmunity and excessive immune responses, but can also mediate detrimental tolerance to tumors and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome (BAC)-transgenic DEREG mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3(+) Tregs selectively express eGFP and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about important considerations such as DT toxicity, which affects any mouse strain treated with DT, and Treg rebound after depletion. Additionally, we point out the specific advantages of BAC-transgenic DEREG mice including their suitability to study organ-specific autoimmunity such as type I diabetes. Moreover, we discuss recent insights into the role of Tregs in viral infections. In summary, DEREG mice are an important tool to study Treg-mediated tolerance and its therapeutic circumvention.
Citation:
Advantages of Foxp3(+) regulatory T cell depletion using DEREG mice. 2014, 2 (3):162-5 Immun Inflamm Dis
Journal:
Immunity, inflammation and disease
Issue Date:
Nov-2014
URI:
http://hdl.handle.net/10033/338558
DOI:
10.1002/iid3.33
PubMed ID:
25505550
Type:
Article
Language:
en
ISSN:
2050-4527
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValue Language
dc.contributor.authorMayer, Christian Ten
dc.contributor.authorLahl, Katharinaen
dc.contributor.authorMilanez-Almeida, Pedroen
dc.contributor.authorWatts, Deepikaen
dc.contributor.authorDittmer, Ulfen
dc.contributor.authorFyhrquist, Nannaen
dc.contributor.authorHuehn, Jochenen
dc.contributor.authorKopf, Manfreden
dc.contributor.authorKretschmer, Karstenen
dc.contributor.authorRouse, Barryen
dc.contributor.authorSparwasser, Timen
dc.date.accessioned2015-01-19T15:57:32Zen
dc.date.available2015-01-19T15:57:32Zen
dc.date.issued2014-11en
dc.identifier.citationAdvantages of Foxp3(+) regulatory T cell depletion using DEREG mice. 2014, 2 (3):162-5 Immun Inflamm Disen
dc.identifier.issn2050-4527en
dc.identifier.pmid25505550en
dc.identifier.doi10.1002/iid3.33en
dc.identifier.urihttp://hdl.handle.net/10033/338558en
dc.description.abstractSeveral mechanisms enable immunological self-tolerance. Regulatory T cells (Tregs) are a specialized T cell subset that prevents autoimmunity and excessive immune responses, but can also mediate detrimental tolerance to tumors and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome (BAC)-transgenic DEREG mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3(+) Tregs selectively express eGFP and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about important considerations such as DT toxicity, which affects any mouse strain treated with DT, and Treg rebound after depletion. Additionally, we point out the specific advantages of BAC-transgenic DEREG mice including their suitability to study organ-specific autoimmunity such as type I diabetes. Moreover, we discuss recent insights into the role of Tregs in viral infections. In summary, DEREG mice are an important tool to study Treg-mediated tolerance and its therapeutic circumvention.en
dc.language.isoenen
dc.titleAdvantages of Foxp3(+) regulatory T cell depletion using DEREG mice.en
dc.typeArticleen
dc.identifier.journalImmunity, inflammation and diseaseen

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