Influence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.

2.50
Hdl Handle:
http://hdl.handle.net/10033/338665
Title:
Influence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.
Authors:
Pisano, Fabio; Heine, Wiebke; Rosenheinrich, Maik; Schweer, Janina; Nuss, Aaron M; Dersch, Petra ( 0000-0001-8177-3280 )
Abstract:
The two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on virulence, lymphatic and systemic organ colonization, and on immune response modulation by IP32953 and YPIII, indicating that PhoP is not absolutely essential for oral infections but may be involved in fine-tuning the outcome. Our work further revealed certain strain-specific differences in virulence properties, which do not strongly rely on the function of PhoP, but affect tissue colonization, dissemination and/or persistence of the bacteria. Highlighted intra-species variations may provide a potential means to rapidly adjust to environmental changes inside and outside of the host.
Citation:
Influence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route. 2014, 9 (7):e103541 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/338665
DOI:
10.1371/journal.pone.0103541
PubMed ID:
25075520
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorPisano, Fabioen
dc.contributor.authorHeine, Wiebkeen
dc.contributor.authorRosenheinrich, Maiken
dc.contributor.authorSchweer, Janinaen
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorDersch, Petraen
dc.date.accessioned2015-01-22T12:24:14Zen
dc.date.available2015-01-22T12:24:14Zen
dc.date.issued2014en
dc.identifier.citationInfluence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route. 2014, 9 (7):e103541 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25075520en
dc.identifier.doi10.1371/journal.pone.0103541en
dc.identifier.urihttp://hdl.handle.net/10033/338665en
dc.description.abstractThe two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on virulence, lymphatic and systemic organ colonization, and on immune response modulation by IP32953 and YPIII, indicating that PhoP is not absolutely essential for oral infections but may be involved in fine-tuning the outcome. Our work further revealed certain strain-specific differences in virulence properties, which do not strongly rely on the function of PhoP, but affect tissue colonization, dissemination and/or persistence of the bacteria. Highlighted intra-species variations may provide a potential means to rapidly adjust to environmental changes inside and outside of the host.en
dc.language.isoenen
dc.titleInfluence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.en
dc.typeArticleen
dc.identifier.journalPloS oneen

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