2.50
Hdl Handle:
http://hdl.handle.net/10033/338727
Title:
Identification of an immune-regulated phagosomal Rab cascade in macrophages.
Authors:
Pei, Gang; Repnik, Urska; Griffiths, Gareth; Gutierrez, Maximiliano Gabriel
Abstract:
Interferon-γ (IFN-γ) has been shown to regulate phagosome trafficking and function in macrophages, but the molecular mechanisms involved are poorly understood. Here, we identify Rab20 as part of the machinery by which IFN-γ controls phagosome maturation. We found that IFN-γ stimulates the association of Rab20 with early phagosomes in macrophages. By using imaging of single phagosomes in live cells, we found that Rab20 induces an early delay in phagosome maturation and extends the time for which Rab5a and phosphatidylinositol 3-phosphate (PI3P) remain associated with phagosomes. Moreover, Rab20 depletion in macrophages abrogates the delay in phagosome maturation induced by IFN-γ. Finally, we demonstrate that Rab20 interacts with the Rab5a guanine nucleotide exchange factor Rabex-5 (also known as RABGEF1) and that Rab20 knockdown impairs the IFN-γ-dependent recruitment of Rabex-5 and Rab5a into phagosomes. Taken together, here, we uncover Rab20 as a key player in the Rab cascade by which IFN-γ induces a delay in phagosome maturation in macrophages.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7 , D-38124 Braunschweig, Germany.
Citation:
Identification of an immune-regulated phagosomal Rab cascade in macrophages. 2014, 127 (Pt 9):2071-82 J. Cell. Sci.
Journal:
Journal of cell science
Issue Date:
1-May-2014
URI:
http://hdl.handle.net/10033/338727
DOI:
10.1242/jcs.144923
PubMed ID:
24569883
Type:
Article
Language:
en
ISSN:
1477-9137
Appears in Collections:
Publications of the RG Phagosomen Biologie

Full metadata record

DC FieldValue Language
dc.contributor.authorPei, Gangen
dc.contributor.authorRepnik, Urskaen
dc.contributor.authorGriffiths, Garethen
dc.contributor.authorGutierrez, Maximiliano Gabrielen
dc.date.accessioned2015-01-23T15:47:36Z-
dc.date.available2015-01-23T15:47:36Z-
dc.date.issued2014-05-01-
dc.identifier.citationIdentification of an immune-regulated phagosomal Rab cascade in macrophages. 2014, 127 (Pt 9):2071-82 J. Cell. Sci.en
dc.identifier.issn1477-9137-
dc.identifier.pmid24569883-
dc.identifier.doi10.1242/jcs.144923-
dc.identifier.urihttp://hdl.handle.net/10033/338727-
dc.description.abstractInterferon-γ (IFN-γ) has been shown to regulate phagosome trafficking and function in macrophages, but the molecular mechanisms involved are poorly understood. Here, we identify Rab20 as part of the machinery by which IFN-γ controls phagosome maturation. We found that IFN-γ stimulates the association of Rab20 with early phagosomes in macrophages. By using imaging of single phagosomes in live cells, we found that Rab20 induces an early delay in phagosome maturation and extends the time for which Rab5a and phosphatidylinositol 3-phosphate (PI3P) remain associated with phagosomes. Moreover, Rab20 depletion in macrophages abrogates the delay in phagosome maturation induced by IFN-γ. Finally, we demonstrate that Rab20 interacts with the Rab5a guanine nucleotide exchange factor Rabex-5 (also known as RABGEF1) and that Rab20 knockdown impairs the IFN-γ-dependent recruitment of Rabex-5 and Rab5a into phagosomes. Taken together, here, we uncover Rab20 as a key player in the Rab cascade by which IFN-γ induces a delay in phagosome maturation in macrophages.en
dc.language.isoenen
dc.subject.meshBlotting, Westernen
dc.subject.meshCell Lineen
dc.subject.meshElectrophoresis, Polyacrylamide Gelen
dc.subject.meshFluorescent Antibody Technique, Indirecten
dc.subject.meshGuanine Nucleotide Exchange Factorsen
dc.subject.meshHumansen
dc.subject.meshImmunoprecipitationen
dc.subject.meshLysosomesen
dc.subject.meshMacrophagesen
dc.subject.meshPhagosomesen
dc.subject.meshPhosphatidylinositol Phosphatesen
dc.subject.meshrab GTP-Binding Proteinsen
dc.subject.meshrab5 GTP-Binding Proteinsen
dc.titleIdentification of an immune-regulated phagosomal Rab cascade in macrophages.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7 , D-38124 Braunschweig, Germany.en
dc.identifier.journalJournal of cell scienceen

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