Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 Fatty acids.

2.50
Hdl Handle:
http://hdl.handle.net/10033/344386
Title:
Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 Fatty acids.
Authors:
Garcia, Ronald; Pistorius, Dominik; Stadler, Marc ( 0000-0002-7284-8671 ) ; Müller, Rolf
Abstract:
In an analysis of 47 aerobic myxobacterial strains, representing 19 genera in suborders Cystobacterineae, Nannocystineae, Sorangiineae, and a novel isolate, "Aetherobacter" SBSr008, an enormously diverse array of fatty acids (FAs) was found. The distribution of straight-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) supports the reported clustering of strains in the phylogenetic tree based on 16S rRNA genes. This finding additionally allows the prediction and assignment of the novel isolate SBSr008 into its corresponding taxon. Sorangiineae predominantly contains larger amounts of SCFA (57 to 84%) than BCFA. On the other hand, Cystobacterineae exhibit significant BCFA content (53 to 90%), with the exception of the genus Stigmatella. In Nannocystineae, the ratio of BCFA and SCFA seems dependent on the taxonomic clade. Myxobacteria could also be identified and classified by using their specific and predominant FAs as biomarkers. Nannocystineae is remarkably unique among the suborders for its absence of hydroxy FAs. After the identification of arachidonic (AA) FA in Phaselicystidaceae, eight additional polyunsaturated fatty acids (PUFAs) belonging to the omega-6 and omega-3 families were discovered. Here we present a comprehensive report of FAs found in aerobic myxobacteria. Gliding bacteria belonging to Flexibacter and Herpetosiphon were chosen for comparative analysis to determine their FA profiles in relation to the myxobacteria.
Citation:
Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 Fatty acids. 2011, 193 (8):1930-42 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10033/344386
DOI:
10.1128/JB.01091-10
PubMed ID:
21317327
Type:
Article
Language:
en
ISSN:
1098-5530
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorGarcia, Ronalden
dc.contributor.authorPistorius, Dominiken
dc.contributor.authorStadler, Marcen
dc.contributor.authorMüller, Rolfen
dc.date.accessioned2015-02-12T13:47:04Zen
dc.date.available2015-02-12T13:47:04Zen
dc.date.issued2011-04en
dc.identifier.citationFatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 Fatty acids. 2011, 193 (8):1930-42 J. Bacteriol.en
dc.identifier.issn1098-5530en
dc.identifier.pmid21317327en
dc.identifier.doi10.1128/JB.01091-10en
dc.identifier.urihttp://hdl.handle.net/10033/344386en
dc.description.abstractIn an analysis of 47 aerobic myxobacterial strains, representing 19 genera in suborders Cystobacterineae, Nannocystineae, Sorangiineae, and a novel isolate, "Aetherobacter" SBSr008, an enormously diverse array of fatty acids (FAs) was found. The distribution of straight-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) supports the reported clustering of strains in the phylogenetic tree based on 16S rRNA genes. This finding additionally allows the prediction and assignment of the novel isolate SBSr008 into its corresponding taxon. Sorangiineae predominantly contains larger amounts of SCFA (57 to 84%) than BCFA. On the other hand, Cystobacterineae exhibit significant BCFA content (53 to 90%), with the exception of the genus Stigmatella. In Nannocystineae, the ratio of BCFA and SCFA seems dependent on the taxonomic clade. Myxobacteria could also be identified and classified by using their specific and predominant FAs as biomarkers. Nannocystineae is remarkably unique among the suborders for its absence of hydroxy FAs. After the identification of arachidonic (AA) FA in Phaselicystidaceae, eight additional polyunsaturated fatty acids (PUFAs) belonging to the omega-6 and omega-3 families were discovered. Here we present a comprehensive report of FAs found in aerobic myxobacteria. Gliding bacteria belonging to Flexibacter and Herpetosiphon were chosen for comparative analysis to determine their FA profiles in relation to the myxobacteria.en
dc.language.isoenen
dc.subject.meshCluster Analysisen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshFatty Acids, Unsaturateden
dc.subject.meshGas Chromatography-Mass Spectrometryen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMyxococcalesen
dc.subject.meshPhylogenyen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSequence Analysis, DNAen
dc.titleFatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 Fatty acids.en
dc.typeArticleen
dc.identifier.journalJournal of bacteriologyen

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