The Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65.

2.50
Hdl Handle:
http://hdl.handle.net/10033/344811
Title:
The Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65.
Authors:
Jurida, Liane; Soelch, Johanna; Bartkuhn, Marek; Handschick, Katja; Müller, Helmut; Newel, Doris; Weber, Axel; Dittrich-Breiholz, Oliver; Schneider, Heike; Bhuju, Sabin; Saul, Vera V; Schmitz, M Lienhard; Kracht, Michael
Abstract:
The inflammatory gene response requires activation of the protein kinase TAK1, but it is currently unknown how TAK1-derived signals coordinate transcriptional programs in the genome. We determined the genome-wide binding of the TAK1-controlled NF-κB subunit p65 in relation to active enhancers and promoters of transcribed genes by chromatin immunoprecipitation sequencing (ChIP-seq) experiments. Out of 35,000 active enhancer regions, 410 H3K4me1-positive enhancers show interleukin 1 (IL-1)-induced H3K27ac and p65 binding. Inhibition of TAK1 or IKK2 or depletion of p65 blocked inducible enhancer activation and gene expression. As exemplified by the CXC chemokine cluster located on chromosome 4, the TAK1-p65 pathway also regulates the recruitment kinetics of the histone acetyltransferase CBP, of NF-κB p50, and of AP-1 transcription factors to both promoters and enhancers. This study provides a high-resolution view of epigenetic changes occurring during the IL-1 response and allows the genome-wide identification of a distinct class of inducible p65 NF-κB-dependent enhancers in epithelial cells.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
The Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65. 2015: Cell Rep
Journal:
Cell reports
Issue Date:
4-Feb-2015
URI:
http://hdl.handle.net/10033/344811
DOI:
10.1016/j.celrep.2015.01.001
PubMed ID:
25660023
Type:
Article
ISSN:
2211-1247
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorJurida, Lianeen
dc.contributor.authorSoelch, Johannaen
dc.contributor.authorBartkuhn, Mareken
dc.contributor.authorHandschick, Katjaen
dc.contributor.authorMüller, Helmuten
dc.contributor.authorNewel, Dorisen
dc.contributor.authorWeber, Axelen
dc.contributor.authorDittrich-Breiholz, Oliveren
dc.contributor.authorSchneider, Heikeen
dc.contributor.authorBhuju, Sabinen
dc.contributor.authorSaul, Vera Ven
dc.contributor.authorSchmitz, M Lienharden
dc.contributor.authorKracht, Michaelen
dc.date.accessioned2015-02-23T13:44:13Zen
dc.date.available2015-02-23T13:44:13Zen
dc.date.issued2015-02-04en
dc.identifier.citationThe Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65. 2015: Cell Repen
dc.identifier.issn2211-1247en
dc.identifier.pmid25660023en
dc.identifier.doi10.1016/j.celrep.2015.01.001en
dc.identifier.urihttp://hdl.handle.net/10033/344811en
dc.description.abstractThe inflammatory gene response requires activation of the protein kinase TAK1, but it is currently unknown how TAK1-derived signals coordinate transcriptional programs in the genome. We determined the genome-wide binding of the TAK1-controlled NF-κB subunit p65 in relation to active enhancers and promoters of transcribed genes by chromatin immunoprecipitation sequencing (ChIP-seq) experiments. Out of 35,000 active enhancer regions, 410 H3K4me1-positive enhancers show interleukin 1 (IL-1)-induced H3K27ac and p65 binding. Inhibition of TAK1 or IKK2 or depletion of p65 blocked inducible enhancer activation and gene expression. As exemplified by the CXC chemokine cluster located on chromosome 4, the TAK1-p65 pathway also regulates the recruitment kinetics of the histone acetyltransferase CBP, of NF-κB p50, and of AP-1 transcription factors to both promoters and enhancers. This study provides a high-resolution view of epigenetic changes occurring during the IL-1 response and allows the genome-wide identification of a distinct class of inducible p65 NF-κB-dependent enhancers in epithelial cells.en
dc.languageENGen
dc.titleThe Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalCell reportsen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.