CD8+ Foxp3+ T cells share developmental and phenotypic features with classical CD4+ Foxp3+ regulatory T cells but lack potent suppressive activity.

2.50
Hdl Handle:
http://hdl.handle.net/10033/346205
Title:
CD8+ Foxp3+ T cells share developmental and phenotypic features with classical CD4+ Foxp3+ regulatory T cells but lack potent suppressive activity.
Authors:
Mayer, Christian T; Floess, Stefan; Baru, Abdul Mannan; Lahl, Katharina; Huehn, Jochen; Sparwasser, Tim ( 0000-0001-5645-902X )
Abstract:
"Suppressor T cells" were historically defined within the CD8(+) T-cell compartment and recent studies have highlighted several naturally occurring CD8(+) Foxp3(-) Treg populations. However, the relevance of CD8(+) Foxp3(+) T cells, which represent a minor population in both thymi and secondary lymphoid organs of nonmanipulated mice, remains unclear. We here demonstrate that de novo Foxp3 induction in peripheral CD8(+) Foxp3(-) T cells is counter-regulated by DC-mediated co-stimulation via CD80/CD86. CD8(+) Foxp3(+) T cells fail to develop in TCR-transgenic mice with Rag1(-/-) background, similar to classical CD4(+) Foxp3(+) Tregs. Notably, both naturally occurring and induced CD8(+) Foxp3(+) T cells express bona fide Treg markers including CD25, GITR, CTLA4 and CD103, and show defective IFN-γ production upon restimulation when compared with their CD8(+) Foxp3(-) counterparts. However, utilizing DEREG transgenic mice for the isolation of Foxp3(+) cells by eGFP reporter expression, we demonstrate that induced CD8(+) Foxp3(+) T cells similar to activated CD8(+) Foxp3(-) T cells only mildly suppress T-cell proliferation and IFN-γ production. We therefore categorize CD8(+) Foxp3(+) T cells as a tightly controlled population sharing certain developmental and phenotypic properties with classical CD4(+) Foxp3(+) Tregs, but lacking potent suppressive activity.
Citation:
CD8+ Foxp3+ T cells share developmental and phenotypic features with classical CD4+ Foxp3+ regulatory T cells but lack potent suppressive activity. 2011, 41 (3):716-25 Eur. J. Immunol.
Journal:
European journal of immunology
Issue Date:
Mar-2011
URI:
http://hdl.handle.net/10033/346205
DOI:
10.1002/eji.201040913
PubMed ID:
21312192
Type:
Article
Language:
en
ISSN:
1521-4141
Appears in Collections:
publications of the division experimentelle Immunologie (EXIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorMayer, Christian Ten
dc.contributor.authorFloess, Stefanen
dc.contributor.authorBaru, Abdul Mannanen
dc.contributor.authorLahl, Katharinaen
dc.contributor.authorHuehn, Jochenen
dc.contributor.authorSparwasser, Timen
dc.date.accessioned2015-03-05T12:16:49Zen
dc.date.available2015-03-05T12:16:49Zen
dc.date.issued2011-03en
dc.identifier.citationCD8+ Foxp3+ T cells share developmental and phenotypic features with classical CD4+ Foxp3+ regulatory T cells but lack potent suppressive activity. 2011, 41 (3):716-25 Eur. J. Immunol.en
dc.identifier.issn1521-4141en
dc.identifier.pmid21312192en
dc.identifier.doi10.1002/eji.201040913en
dc.identifier.urihttp://hdl.handle.net/10033/346205en
dc.description.abstract"Suppressor T cells" were historically defined within the CD8(+) T-cell compartment and recent studies have highlighted several naturally occurring CD8(+) Foxp3(-) Treg populations. However, the relevance of CD8(+) Foxp3(+) T cells, which represent a minor population in both thymi and secondary lymphoid organs of nonmanipulated mice, remains unclear. We here demonstrate that de novo Foxp3 induction in peripheral CD8(+) Foxp3(-) T cells is counter-regulated by DC-mediated co-stimulation via CD80/CD86. CD8(+) Foxp3(+) T cells fail to develop in TCR-transgenic mice with Rag1(-/-) background, similar to classical CD4(+) Foxp3(+) Tregs. Notably, both naturally occurring and induced CD8(+) Foxp3(+) T cells express bona fide Treg markers including CD25, GITR, CTLA4 and CD103, and show defective IFN-γ production upon restimulation when compared with their CD8(+) Foxp3(-) counterparts. However, utilizing DEREG transgenic mice for the isolation of Foxp3(+) cells by eGFP reporter expression, we demonstrate that induced CD8(+) Foxp3(+) T cells similar to activated CD8(+) Foxp3(-) T cells only mildly suppress T-cell proliferation and IFN-γ production. We therefore categorize CD8(+) Foxp3(+) T cells as a tightly controlled population sharing certain developmental and phenotypic properties with classical CD4(+) Foxp3(+) Tregs, but lacking potent suppressive activity.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAntigens, CD28en
dc.subject.meshAntigens, CD80en
dc.subject.meshAntigens, CD86en
dc.subject.meshCD8-Positive T-Lymphocytesen
dc.subject.meshCell Differentiationen
dc.subject.meshCell Proliferationen
dc.subject.meshDendritic Cellsen
dc.subject.meshForkhead Transcription Factorsen
dc.subject.meshIn Vitro Techniquesen
dc.subject.meshInterferon-gammaen
dc.subject.meshLymphocyte Activationen
dc.subject.meshMaleen
dc.subject.meshMiceen
dc.subject.meshMice, Knockouten
dc.subject.meshMice, Transgenicen
dc.subject.meshPhenotypeen
dc.subject.meshReceptors, Antigen, T-Cellen
dc.subject.meshSignal Transductionen
dc.subject.meshT-Lymphocyte Subsetsen
dc.subject.meshT-Lymphocytes, Regulatoryen
dc.subject.meshTransforming Growth Factor betaen
dc.titleCD8+ Foxp3+ T cells share developmental and phenotypic features with classical CD4+ Foxp3+ regulatory T cells but lack potent suppressive activity.en
dc.typeArticleen
dc.identifier.journalEuropean journal of immunologyen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.