The M1 protein of Streptococcus pyogenes triggers an innate uptake mechanism into polarized human endothelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/346847
Title:
The M1 protein of Streptococcus pyogenes triggers an innate uptake mechanism into polarized human endothelial cells.
Authors:
Ochel, Anja; Rohde, Manfred; Chhatwal, Gursharan S; Talay, Susanne R
Abstract:
Serotype M1 Streptococcus pyogenes is a major human pathogen associated with severe invasive diseases causing high morbidity and mortality. In a substantial number of cases, invasive disease develops in previously healthy individuals with no obvious port of entry. This has led to the hypothesis that the source of streptococci in these cases is a transient bacteraemia. This study focuses on the analysis of interaction of tissue-invasive serotype M1 S. pyogenes with human endothelial cells (EC) of the vascular system. We identify the M1 surface protein of S. pyogenes as the EC invasin which is recognised by polarized human blood EC, thereby triggering rapid, phagocytosis-like uptake of streptococci into polarized EC layers. Upon internalization, the M1 S. pyogenes serotype is incorporated into phagosomes which traffic via the endosomal/lysosomal pathway. However, some of the streptococci successfully evade this innate killing process and hereby mediate their escape into the cytoplasm of the host cell. The results of this study demonstrate that blood EC possess an efficient uptake mechanism for serotype M1 S. pyogenes. Despite efficient phagocytosis, streptococcal survival within EC constitutes one potential mechanism which favours intracellular persistence and thus facilitates continuous infection and dissemination from the primary side of infection into deep tissue.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweíg, Germany.
Citation:
The M1 protein of Streptococcus pyogenes triggers an innate uptake mechanism into polarized human endothelial cells. 2014, 6 (5):585-96 J Innate Immun
Journal:
Journal of innate immunity
Issue Date:
2014
URI:
http://hdl.handle.net/10033/346847
DOI:
10.1159/000358085
PubMed ID:
24504091
Type:
Article
Language:
en
ISSN:
1662-8128
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorOchel, Anjaen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorChhatwal, Gursharan Sen
dc.contributor.authorTalay, Susanne Ren
dc.date.accessioned2015-03-18T15:19:41Zen
dc.date.available2015-03-18T15:19:41Zen
dc.date.issued2014en
dc.identifier.citationThe M1 protein of Streptococcus pyogenes triggers an innate uptake mechanism into polarized human endothelial cells. 2014, 6 (5):585-96 J Innate Immunen
dc.identifier.issn1662-8128en
dc.identifier.pmid24504091en
dc.identifier.doi10.1159/000358085en
dc.identifier.urihttp://hdl.handle.net/10033/346847en
dc.description.abstractSerotype M1 Streptococcus pyogenes is a major human pathogen associated with severe invasive diseases causing high morbidity and mortality. In a substantial number of cases, invasive disease develops in previously healthy individuals with no obvious port of entry. This has led to the hypothesis that the source of streptococci in these cases is a transient bacteraemia. This study focuses on the analysis of interaction of tissue-invasive serotype M1 S. pyogenes with human endothelial cells (EC) of the vascular system. We identify the M1 surface protein of S. pyogenes as the EC invasin which is recognised by polarized human blood EC, thereby triggering rapid, phagocytosis-like uptake of streptococci into polarized EC layers. Upon internalization, the M1 S. pyogenes serotype is incorporated into phagosomes which traffic via the endosomal/lysosomal pathway. However, some of the streptococci successfully evade this innate killing process and hereby mediate their escape into the cytoplasm of the host cell. The results of this study demonstrate that blood EC possess an efficient uptake mechanism for serotype M1 S. pyogenes. Despite efficient phagocytosis, streptococcal survival within EC constitutes one potential mechanism which favours intracellular persistence and thus facilitates continuous infection and dissemination from the primary side of infection into deep tissue.en
dc.language.isoenen
dc.titleThe M1 protein of Streptococcus pyogenes triggers an innate uptake mechanism into polarized human endothelial cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweíg, Germany.en
dc.identifier.journalJournal of innate immunityen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.